Nocodazole, Microtubule Inhibitor / CRISPR Editing Enhancer

Nocodazole is a potent microtubule inhibitor to inhibit mitosis. It disrupts microtubules by binding to β−tubulin and preventing formation of one of the two interchain disulfide linkages, thus inhibiting microtubule dynamics, disruption of mitotic spindle function, and fragmentation of the Golgi complex. Nocodazole arrests the cell cycle at G2/M phase and also prevents phosphorylation of the T cell antigen receptor and inhibits its activity. Nocodazole stimulates the intrinsic GTPase activity of tubulin and activates the JNK/SAPK signaling pathway and induces apoptosis in several normal and tumor cell lines. Nocodazole enhances homology-directed repair (HDR) efficiency 9 to 31% (depending on cell cycle phase) and increases Cas9-mediated gene editing frequencies.

Product information

CAS Number: 31430-18-9

Molecular Weight: 301.32

Formula: C14H11N3O3S

Chemical Name: methyl (5-(thiophene-2-carbonyl)-1H-benzo[d]imidazol-2-yl)carbamate

Smiles: COC(=O)NC1NC2=CC=C(C=C2N=1)C(=O)C1=CC=CS1

InChiKey: KYRVNWMVYQXFEU-UHFFFAOYSA-N

InChi: InChI=1S/C14H11N3O3S/c1-20-14(19)17-13-15-9-5-4-8(7-10(9)16-13)12(18)11-3-2-6-21-11/h2-7H,1H3,(H2,15,16,17,19)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO up to 50 mM

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

Nocodazole was used at 200 ng/ml in vitro and cellular assays.

References:

1. Vasquez RJ, et al. Nanomolar concentrations of nocodazole alter microtubule dynamic instability in vivo and in vitro. (1997) Mol Biol Cell. 8(6):973-85.
2. Webb JL, et al. Microtubule disruption inhibits autophagosome-lysosome fusion: implications for studying the roles of aggresomes in polyglutamine diseases. (2004) Int J Biochem Cell Biol. 36(12):2541-50.
3. Mizushima N, et al. Methods in mammalian autophagy research. (2010) Cell. 140(3):313-26.

Products are for research use only. Not for human use.

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