Sp-8-CPT-cAMPS

Sp-8-CPT-cAMPS, a cAMP analog, is a potent and selective activator of the cAMP-dependent protein kinas A (PKA I and PKA II). Sp-8-CPT-cAMPS selects site A of RI compares to site A of RII by 153-fold and site B of RII compares to site B of RI by 59-fold.

Product information

CAS Number: 129693-13-6

Molecular Weight: 487.88

Formula: C16H15ClN5O5PS2

Chemical Name: (2S,4aR,6R,7R,7aS)-6-{6-amino-8-[(4-chlorophenyl)sulfanyl]-9H-purin-9-yl}-4a,6,7a-trihydrogenio-7-hydroxy-2-sulfanyl-hexahydro-2λ⁵-furo[3,2-d][1,3,2]dioxaphosphinin-2-one

Smiles: NC1N=CN=C2C=1N=C(SC1C=CC(Cl)=CC=1)N2[C@@H]1O[C@@H]2CO[P@](=O)(S)O[C@H]2[C@H]1O

InChiKey: IVNQJYQKSYRLTE-UDBHDVSKSA-N

InChi: InChI=1S/C16H15ClN5O5PS2/c17-7-1-3-8(4-2-7)30-16-21-10-13(18)19-6-20-14(10)22(16)15-11(23)12-9(26-15)5-25-28(24,29)27-12/h1-4,6,9,11-12,15,23H,5H2,(H,24,29)(H2,18,19,20)/t9-,11-,12-,15-,28+/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Sp-8-CPT-cAMPS (100 μM; 24 h) enhances the IL-1β-stimulated nitrite release, and increases the release ofnitrite by vascular smooth muscle cells by 3 fold in the absence of IL-1β in vascular smooth muscle cells. Sp-8-CPT-cAMPS (100 μM; 24 h) increases IL-1β-induced expression of iNOS protein in rat aortic smooth muscle cells. Sp-8-CPT-cAMPS (10 μM; 30 min) exhibits anti-spasmogenic activity on ACh-induced tension development in guinea-pig trachealis.

References:

1. Dostmann WR , et, al. Probing the cyclic nucleotide binding sites of cAMP-dependent protein kinases I and II with analogs of adenosine 3',5'-cyclic phosphorothioates. J Biol Chem. 1990 Jun 25;265(18):10484-91.
2. Boese M, et, al. Effect of cyclic GMP-dependent vasodilators on the expression of inducible nitric oxide synthase in vascular smooth muscle cells: role of cyclic AMP. Br J Pharmacol. 1996 Oct;119(4):707-15.
3. Spicuzza L, et, al. Evidence that the anti-spasmogenic effect of the beta-adrenoceptor agonist, isoprenaline, on guinea-pig trachealis is not mediated by cyclic AMP-dependent protein kinase. Br J Pharmacol. 2001 Aug;133(8):1201-12.

Products are for research use only. Not for human use.