Description
Hoechst 33342 analog trihydrochloride is an anglog of Hoechst 33342, which is a DNA minor groove binder used fluorochrome for visualizing cellular DNA.
Product information
CAS Number: 159277-19-7
Molecular Weight: 699.97
Formula: C32H40Cl5N7
Chemical Name: N,N-bis(2-chloroethyl)-4-(3-{6-[6-(4-methylpiperazin-1-yl)-1H-1,3-benzodiazol-2-yl]-1H-1,3-benzodiazol-2-yl}propyl)aniline; tris(chlorohydrogen)
Smiles: Cl.Cl.Cl.CN1CCN(CC1)C1=CC2NC(=NC=2C=C1)C1=CC2NC(CCCC3C=CC(=CC=3)N(CCCl)CCCl)=NC=2C=C1
InChiKey: PLUDBJRFRHMTGA-UHFFFAOYSA-N
InChi: InChI=1S/C32H37Cl2N7.3ClH/c1-39-17-19-41(20-18-39)26-10-12-28-30(22-26)38-32(37-28)24-7-11-27-29(21-24)36-31(35-27)4-2-3-23-5-8-25(9-6-23)40(15-13-33)16-14-34;;;/h5-12,21-22H,2-4,13-20H2,1H3,(H,35,36)(H,37,38);3*1H
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Hoechst 33342 binds to adenine-thymine-rich regions of DNA in the minor groove. Hoechst 33342 labels nuclear DNA of cells grown in culture. Image the cells (λex~353 nm, λem~483 nm for Hoechst 33342).
References:
- Chazotte B. Labeling nuclear DNA with hoechst 33342. Cold Spring Harb Protoc. 2011 Jan 1;2011(1):pdb.prot5557.
Products are for research use only. Not for human use.
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