Description
KKL-10 is a small-molecule ribosome rescue inhibitor with broad-spectrum antimicrobial activity against bacteria.
Product information
CAS Number: 952849-76-2
Molecular Weight: 364.22
Formula: C14H10BrN3O2S
Chemical Name: 5-bromo-N-[5-(4-methylphenyl)-1,3,4-oxadiazol-2-yl]thiophene-2-carboxamide
Smiles: CC1C=CC(=CC=1)C1=NN=C(NC(=O)C2=CC=C(Br)S2)O1
InChiKey: QKSDWSBGDVYRKQ-UHFFFAOYSA-N
InChi: InChI=1S/C14H10BrN3O2S/c1-8-2-4-9(5-3-8)13-17-18-14(20-13)16-12(19)10-6-7-11(15)21-10/h2-7H,1H3,(H,16,18,19)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 2.78 mg/mL (7.63 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
The ribosome rescue inhibitor KKL-10 exhibits exceptional antimicrobial activity against both attenuated and fully virulent strains of F. tularensis. The minimum inhibitory concentration (MIC) against F. tularensis strain LVS and Schu S4 are 0.12 and 0.48 μg/mL, respectively. KKL-10 arrests intracellular growth of F. tularensis during all stages of infection. KKL-10 does not affect macrophage viability or function. KKL-10 produces cytotoxic effects of less than 5% at concentrations up to 17.5 μg/mL. The combination of IFN-γ stimulation and KKL-10 activity results in a reduction of the bacterial load by >99.9%. KKL-10 is also able to inhibit growth of F. tularensis inside eukaryotic cells and show no toxicity to HepG2 cells.
References:
- Goralski TD, et al. Inhibitors of Ribosome Rescue Arrest Growth of Francisella tularensis at All Stages of Intracellular Replication. Antimicrob Agents Chemother. 2016 May 23;60(6):3276-82.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.