Description
L-Ascorbic acid 2-phosphate magnesium (2-Phospho-L-ascorbic acid magnesium) is a long-acting vitamin C derivative that can stimulate collagen formation and expression. L-Ascorbic acid 2-phosphate magnesium (2-Phospho-L-ascorbic acid magnesium) can be used as a culture medium supplement for the osteogenic differentiation of human adipose stem cells (hASCs). L-Ascorbic acid 2-phosphate magnesium (2-Phospho-L-ascorbic acid magnesium) increases alkaline phosphatase (ALP) activity and expression of runx2A in hASCs during the osteogenic differentiation.
Product information
CAS Number: 113170-55-1
Molecular Weight: 307.55
Formula: C12H16Mg3O20P2
Chemical Name: trimagnesium(2+) bis((2Z,4R,5S)-3,4,5,6-tetrahydroxy-2-(phosphonatooxy)hex-2-enoate)
Smiles: [Mg+2].[Mg+2].[Mg+2].[O-]P([O-])(=O)O/C(=C(\O)/[C@H](O)[C@@H](O)CO)/C([O-])=O.[O-]P([O-])(=O)O/C(=C(\O)/[C@H](O)[C@@H](O)CO)/C([O-])=O
InChiKey: UMHSVFRRVSZMHT-JONHJXJISA-H
InChi: InChI=1S/2C6H11O10P.3Mg/c2*7-1-2(8)3(9)4(10)5(6(11)12)16-17(13,14)15;;;/h2*2-3,7-10H,1H2,(H,11,12)(H2,13,14,15);;;/q;;3*+2/p-6/b2*5-4-;;;/t2*2-,3+;;;/m00.../s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
L-Ascorbic acid 2-phosphate (0.1-1.5 mM; 2 to 3 weeks with medium exchange every 2 to 3 days) significantly stimulates cell growth, whereas addition of l-Ascorbic acid (Asc) achieves only weak growth stimulation. A combination of Asc-2P and bFGF significantly increases cell growth, but supplementation with EGF and/or insulin does not have any additional effect . L-Ascorbic acid 2-phosphate (50 µM-250 µM) is needed for the effective osteogenic differentiation of human adipose stem cells (hASCs), and higher concentrations of AsA2-P results in increased runx2 expression and ALP activity. The highest proliferation, ALP activity and runx2 expression is achieved with 150 µM AsA2-P and 10 nM dexamethasone (Dex), and 250 µM AsA2-P and 5 nM Dex.
Products are for research use only. Not for human use.
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