Description
DFHBI-1T is a membrane-permeable RNA aptamers-activated fluorescence probe (ex/em=472 nm/507 nm). DFHBI-1T binds to RNA aptamers (Spinach, Spinach2, iSpinach, and Broccoli) and causes specific fluorescence and lower background fluorescence. DFHBI-1T is used to image RNA in live cells.
Product information
CAS Number: 1539318-36-9
Molecular Weight: 320.21
Formula: C13H9F5N2O2
Chemical Name: (4Z)-4-[(3,5-difluoro-4-hydroxyphenyl)methylidene]-2-methyl-1-(2,2,2-trifluoroethyl)-4,5-dihydro-1H-imidazol-5-one
Smiles: CC1=N/C(=C\C2=CC(F)=C(O)C(F)=C2)/C(=O)N1CC(F)(F)F
InChiKey: AWYCLBWNRONMQC-WMZJFQQLSA-N
InChi: InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4-
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 50 mg/mL (156.15 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
DFHBI-1T (20 μM; for 10 min) increases fluorescence in COS7 cells expressing (CGG)60-Spinach2 over DFHBI (20 μM). Broccoli-DFHBI-1T has ex/em=472 nm/507 nm and Spinach2-DFHBI-1T has ex/em=482 nm/505 nm.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.