2-(Methylamino)-1H-purin-6(7H)-one


Catalog No. Size PriceQuantity
M16560-C Contact sales@xcessbio.com for quotation $100Unavailable

Description

2-(Methylamino)-1H-purin-6(7H)-one (N2-Methylguanine) is a modified nucleoside. 2-(Methylamino)-1H-purin-6(7H)-one is an endogenous methylated nucleoside found in human fluids.

Product information

CAS Number: 10030-78-1

Molecular Weight: 165.15

Formula: C6H7N5O

Chemical Name: 2-(methylamino)-6,7-dihydro-3H-purin-6-one

Smiles: CNC1NC2N=CNC=2C(=O)N=1

InChiKey: SGSSKEDGVONRGC-UHFFFAOYSA-N

InChi: InChI=1S/C6H7N5O/c1-7-6-10-4-3(5(12)11-6)8-2-9-4/h2H,1H3,(H3,7,8,9,10,11,12)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 1 mg/mL (6.06 mM; Need ultrasonic). H2O : 1 mg/mL (6.06 mM; ultrasonic and warming and heat to 80°C).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) is found within both helical and looped regions of RNA secondary structure, and it can exist in either the s-cis or the s-trans rotamer. If there is a rotational preference for the methyl group, the effect of 2-(Methylamino)-1H-purin-6(7H)-one substitution may be specific to the sequence context depending upon which face of the base participates in hydrogen bonding. 2-(Methylamino)-1H-purin-6(7H)-one (N2-methylguanine) is the principal kinetic barrier for reverse transcription in the 1340 bases proximal to the 5' end of E. coli 16S rRNA. Transcription intermediates correspond to attenuation at the positions of 2-(Methylamino)-1H-purin-6(7H)-one in the rRNA sequence. The relaxation time for elongation of the cDNA through m2G is approximately 3 min.

Products are for research use only. Not for human use.

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