Description
FM4-64, a membrane-selective red fluorescent dye, belongs to the FM family of styrylpyridinium dyes. FM4-64 is widely used to study endocytosis and exocytosis, vesicle trafficking and organelle organization in living animal.
Product information
CAS Number: 162112-35-8
Molecular Weight: 607.51
Formula: C30H45Br2N3
Chemical Name: 4-[(1E,3E,5E)-6-[4-(diethylamino)phenyl]hexa-1,3,5-trien-1-yl]-1-[3-(triethylazaniumyl)propyl]pyridin-1-ium dibromide
Smiles: [Br-].[Br-].CC[N+](CCC[N+]1C=CC(/C=C/C=C/C=C/C2C=CC(=CC=2)N(CC)CC)=CC=1)(CC)CC
InChiKey: AFVSZGYRRUMOFH-UHFFFAOYSA-L
InChi: InChI=1S/C30H45N3.2BrH/c1-6-32(7-2)30-20-18-28(19-21-30)16-13-11-12-14-17-29-22-25-31(26-23-29)24-15-27-33(8-3,9-4)10-5;;/h11-14,16-23,25-26H,6-10,15,24,27H2,1-5H3;2*1H/q+2;;/p-2
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
FM4-64 readily translocates to the nuclear envelope (NE) at physiological temperature where it exhibits enhanced fluorescence when excited at 620-650 nm in contrast to 480-520 nm excitation in the endocytic pathway and in the endoplasmic reticulum (ER). In vitro data indicated that the dye reveals an enrichment of negatively charged lipids, presumably due to local phospholipid synthesis. Dual-excitation imaging of FM4-64 in relation to lamina-associated polypeptide-1-green fluorescent protein during mitosis suggested that the disassembly of NE preserves microscale lipid complexes in the ER. For studies onplant and fungal cells, FM4-64 is usually preferred to FM1-43 due to its superior brightness, greater contrast, higher photostability, and its red-shifted emission facilitating concomitantuse with the green fluorescent protein (GFP).
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.