Abz-FRLKGGAPIKGV-EDDNP TFA

Abz-FRLKGGAPIKGV-EDDNP TFA is a fluorogenic substrate used to measure the enzymatic activities of protease forms, such as papain-like protease 2 (PLP2) from severe acute respiratory syndrome coronavirus (SARS-CoV). Abz-FRLKGGAPIKGV-EDDNP TFA has the potential for study 2019-nCoV infection.

Product information

Molecular Weight: 2046.16

Formula: C89H135F3N24O28

Chemical Name: (4-aminobenzoyl)-L-phenylalanyl-L-arginyl-L-leucyl-L-lysylglycylglycyl-L-alanyl-L-prolyl-L-isoleucyl-L-lysylglycyl-L-valyl-L-glutamyl-L-aspartyl-L-aspartyl-L-asparaginyl-L-proline--2, 2, 2-trifluoroacetic acid (1/1)

Smiles: CC[C@H](C)[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](C)NC(=O)CNC(=O)CNC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(N)=N)NC(=O)[C@H](CC1C=CC=CC=1)NC(=O)C1C=CC(N)=CC=1)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(O)=O.OC(=O)C(F)(F)F

InChiKey: ZCFYIJFMZYQGAZ-VWMPMTHHSA-N

InChi: InChI=1S/C87H134N24O26.C2HF3O2/c1-8-47(6)71(83(133)101-53(22-13-15-33-89)74(124)97-44-66(115)108-70(46(4)5)82(132)102-55(30-31-67(116)117)76(126)105-58(40-68(118)119)79(129)106-59(41-69(120)121)80(130)107-60(39-63(91)112)85(135)111-36-18-25-62(111)86(136)137)109-81(131)61-24-17-35-110(61)84(134)48(7)98-65(114)43-95-64(113)42-96-73(123)52(21-12-14-32-88)99-77(127)56(37-45(2)3)104-75(125)54(23-16-34-94-87(92)93)100-78(128)57(38-49-19-10-9-11-20-49)103-72(122)50-26-28-51(90)29-27-50;3-2(4,5)1(6)7/h9-11,19-20,26-29,45-48,52-62,70-71H,8,12-18,21-25,30-44,88-90H2,1-7H3,(H2,91,112)(H,95,113)(H,96,123)(H,97,124)(H,98,114)(H,99,127)(H,100,128)(H,101,133)(H,102,132)(H,103,122)(H,104,125)(H,105,126)(H,106,129)(H,107,130)(H,108,115)(H,109,131)(H,116,117)(H,118,119)(H,120,121)(H,136,137)(H4,92,93,94);(H,6,7)/t47-,48-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,70-,71-;/m0./s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Abz-FRLKGGAPIKGV-EDDNP TFA has little fluorescent emission because of the fluorescence resonance energy transfer (FRET) between Abz and EDDNP. The apparent kcat and Km values for this fluorogenic substrate at pH 7.4 are 20 min-1 and 77 µM, respectively. The enhanced fluorescence emission upon substrate cleavage is monitored at the excitation and emission wavelengths of 320 and 420 nm, respectively, in the presence or absence of the chemical compounds assayed.

Products are for research use only. Not for human use.