Description
Xylopine is an aporphine alkaloid with cytotoxic activity on cancer cells. Xylopine induces oxidative stress, causes G2/M cell cycle arrest and apoptosis in cancer cells.
Product information
CAS Number: 517-71-5
Molecular Weight: 295.33
Formula: C18H17NO3
Chemical Name: (12R)-16-methoxy-3, 5-dioxa-11-azapentacyclo[10.7.1.02, 6.08, 20.014, 19]icosa-1(20), 2(6), 7, 14(19), 15, 17-hexaene
Smiles: COC1=CC2C[C@H]3NCCC4=CC5OCOC=5C(=C43)C=2C=C1
InChiKey: RFWCCZDSXIZJMF-CQSZACIVSA-N
InChi: InChI=1S/C18H17NO3/c1-20-12-2-3-13-11(6-12)7-14-16-10(4-5-19-14)8-15-18(17(13)16)22-9-21-15/h2-3,6,8,14,19H,4-5,7,9H2,1H3/t14-/m1/s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Xylopine (3.5 μM-14 μM; 24-48 hours) displays potent cytotoxicity in a time- and does-depenpent manner. Xylopine (72 h) has cytotoxic activity, with IC50 values ranging from 6.4 to 26.6 μM in eight different cancer cell lines (MCF7, HCT116, HepG2, SCC-9, HSC-3, HL-60, K-562, and B16-F10). Xylopine (3.5 μM-14 μM; 24-48 hours) causes cell cycle block at the phase G2/M, which is followed by internucleosomal DNA fragmentation. Xylopine (3.5 μM-14 μM; 24-48 hours) significantly increases the early and late apoptosis, induces mitochondrial depolarization, and increases caspase-3 activation. Xylopine also causes an increase in the production of reactive oxygen/nitrogen species (ROS/RNS), including hydrogen peroxide and nitric oxide, but not superoxide anion, and reduces glutathione levels are decreased in Xylopine-treated HCT116 cells. HCT116 cells 3.5 μM, 7 μM, and 14 μM 24 hours, 48 hours Induced G2/M phase arrest. HCT116 cells 3.5 μM, 7 μM, and 14 μM 24 hours, 48 hours Significantly increased the early and late apoptosis.
Products are for research use only. Not for human use.
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