RFRP-3(human)

RFRP-3 (Neuropeptide VF(124-131))(human), a human GnIH peptide homolog, is a potent inhibitor of gonadotropin secretion by inhibiting Ca2+ mobilization. RFRP-3(human) is a NPFF1 receptor agonist, it inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM.

Product information

CAS Number: 311309-27-0

Molecular Weight: 969.14

Formula: C45H72N14O10

Chemical Name: (Z,2S)-2-[(Z)-{[(2S)-1-[(2S)-2-[(Z)-[(2S)-2-[(Z)-{[(2S)-1-[(2S)-2-amino-3-methylbutanoyl]pyrrolidin-2-yl](hydroxy)methylidene}amino]-1-hydroxy-3-(C-hydroxycarbonimidoyl)propylidene]amino]-4-methylpentanoyl]pyrrolidin-2-yl](hydroxy)methylidene}amino]-N-[(1S)-4-carbamimidamido-1-[(Z)-[(1S)-1-(C-hydroxycarbonimidoyl)-2-phenylethyl]-C-hydroxycarbonimidoyl]butyl]pentanediimidic acid

Smiles: CC(C)C[C@H](/N=C(\O)/[C@H](CC(=N)O)/N=C(\O)/[C@@H]1CCCN1C(=O)[C@@H](N)C(C)C)C(=O)N1CCC[C@H]1/C(/O)=N/[C@@H](CCC(=N)O)/C(/O)=N/[C@@H](CCCNC(N)=N)/C(/O)=N/[C@@H](CC1C=CC=CC=1)C(=N)O

InChiKey: JFZCLMZUABKABL-GVCDGELBSA-N

InChi: InChI=1S/C45H72N14O10/c1-24(2)21-31(57-40(65)30(23-35(47)61)56-42(67)33-15-10-20-59(33)44(69)36(48)25(3)4)43(68)58-19-9-14-32(58)41(66)54-28(16-17-34(46)60)39(64)53-27(13-8-18-52-45(50)51)38(63)55-29(37(49)62)22-26-11-6-5-7-12-26/h5-7,11-12,24-25,27-33,36H,8-10,13-23,48H2,1-4H3,(H2,46,60)(H2,47,61)(H2,49,62)(H,53,64)(H,54,66)(H,55,63)(H,56,67)(H,57,65)(H4,50,51,52)/t27-,28-,29-,30-,31-,32-,33-,36-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

RFRP-3 efficiently inhibits forskolin-induced production of cAMP with an IC50 of 0.7 nM. Scatchard-plot analysis shows that 125I-labelled hRFRP-3 has a single class of high-affinity binding sites for the membrane fractions of CHO cells expressing rat OT7T022, the Kd value and the Bmax values are 0.19 nM and 1.3 pM, respectively. RFRP-3 specifically stimulate cells transfected with a new orphan 7TMR, OT7T022, it binds to OT7T022 as a specific ligand with high affinity (Kd= 0.19 nM). RFRP-3 (10-8 to 10-14M) has no effect on LH and FSH levels alone, but when it combines with GnRH, LH and FSH secretion is significantly reduced by the combination.

Products are for research use only. Not for human use.