3-Methyladenine

3-Methyladenine (3-MA) is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K.

Product information

CAS Number: 5142-23-4

Molecular Weight: 149.15

Formula: C6H7N5

Chemical Name: 3-methyl-3H-purin-6-amine

Smiles: CN1C=NC(N)=C2N=CN=C12

InChiKey: FSASIHFSFGAIJM-UHFFFAOYSA-N

InChi: InChI=1S/C6H7N5/c1-11-3-10-5(7)4-6(11)9-2-8-4/h2-3H,7H2,1H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 20.83 mg/mL (139.66 mM; Need ultrasonic). H2O : 5 mg/mL (33.52 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner. 3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions. 3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy. 3-Methyladenine induces cell death is independent of autophagy inhibition. 3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest. Note: The recommended concentration of 3-MA is approximately 0.5-10 mM in cell culture. DMSO stock solution is not recommended. We suggest that you can weigh out the amount of 3-MA you required before your experiment. Then please dissolve it in medium and sterilize with a 0.22 μm filter.

In Vivo:

3-Methyladenine (1.5 mg/100 g; intraperitoneal injection; 3-24 hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24 hours. 3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP. 3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-κB signaling pathway in sodium taurocholate-induced SAP.

Products are for research use only. Not for human use.