Description
Phen-DC3 Trifluoromethanesulfonate is a G-quadruplex (G4) specific ligand which can inhibit FANCJ and DinG helicases with IC50s of 65±6 and 50±10 nM, respectively.
Product information
CAS Number: 929895-45-4
Molecular Weight: 848.75
Formula: C36H26F6N6O8S2
Chemical Name: 1-methyl-3-{9-[(1-methylquinolin-1-ium-3-yl)carbamoyl]-1,10-phenanthroline-2-amido}quinolin-1-ium ditrifluoromethanesulfonate
Smiles: C[N+]1C=C(C=C2C=CC=CC=12)NC(=O)C1=CC=C2C=CC3=CC=C(N=C3C2=N1)C(=O)NC1=C[N+](C)=C2C=CC=CC2=C1.[O-]S(=O)(=O)C(F)(F)F.[O-]S(=O)(=O)C(F)(F)F
InChiKey: PYJCATLYPXPYHF-UHFFFAOYSA-N
InChi: InChI=1S/C34H24N6O2.2CHF3O3S/c1-39-19-25(17-23-7-3-5-9-29(23)39)35-33(41)27-15-13-21-11-12-22-14-16-28(38-32(22)31(21)37-27)34(42)36-26-18-24-8-4-6-10-30(24)40(2)20-26;2*2-1(3,4)8(5,6)7/h3-20H,1-2H3;2*(H,5,6,7)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : ≥ 34 mg/mL (40.06 mM).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
In WT cells, a CEB1-WT array is rather stable but undergoes frequent rearrangements upon addition of 10 μM Phen-DC3 Trifluoromethanesulfonate (Phen-DC3). It is found that the c-Myc allele exhibits significant destabilization upon Phen-DC3 Trifluoromethanesulfonate treatment and PIF1 deletion. The CEB25-L111(T) array is stable in WT cells, it becomes unstable upon addition of Phen-DC3 Trifluoromethanesulfonate or deletion of PIF1. It is also highly destabilized in the presence of Phen-DC3 Trifluoromethanesulfonate or in the absence of PIF1. The CEB1-loop CEB25 allele remaines fully stable in both PIF1-treated and WT cells.
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