Cholesterol

Cholesterol is the major sterol in mammals and is makes up 20-25% of structural component of the plasma membrane. Plasma membranes are highly permeable to water but relatively impermeable to ions and protons. Cholesterol plays an important role in determining the fluidity and permeability characteristics of the membrane as well as the function of both the transporters and signaling proteins. Cholesterol is also an endogenous estrogen-related receptor α (ERRα) agonist.

Product information

CAS Number: 57-88-5

Molecular Weight: 386.65

Formula: C27H46O

Chemical Name: 9a,11a-dimethyl-1-(6-methylheptan-2-yl)-1H,2H,3H,3aH,3bH,4H,6H,7H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-7-ol

Smiles: CC(C)CCCC(C)C1CCC2C3CC=C4CC(O)CCC4(C)C3CCC12C

InChiKey: HVYWMOMLDIMFJA-UHFFFAOYSA-N

InChi: InChI=1S/C27H46O/c1-18(2)7-6-8-19(3)23-11-12-24-22-10-9-20-17-21(28)13-15-26(20,4)25(22)14-16-27(23,24)5/h9,18-19,21-25,28H,6-8,10-17H2,1-5H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 0.79 mg/mL (2.04 mM; Need warming).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

GT1-7 hypothalamic cells subjected to Cholesterol depletion in vitro produced 20-31% reductions in cellular Cholesterol content. All Cholesterol-depleted neuron-derived cells, exhibit decreased phosphorylation/activation of IRS-1 and AKT following stimulation by insulin, insulin-like growth factor-1, or the neurotrophins (NGF and BDNF). Reduction in cellular Cholesterol also results in increased basal autophagy and impairment of induction of autophagy by glucose deprivation.

In Vivo:

Insulin-deficient diabetes in mice can lead to a reduction in brain Cholesterol synthesis, which occurs through a change in expression of Cholesterol synthesis enzymes and their upstream regulators SREBP2 and SCAP1. The pool of Cholesterol in the whole animal is 2,200 mg/kg body weight. This is true for essentially all species from the mouse to the primate and indicates that the average concentration of Cholesterol in the whole animal is 2.2 mg/g fresh tissue. The basal metabolic rate in the mouse is 170 kilocalories (kcal)/day/kg, and the flow of Cholesterol from all peripheral organs to the liver is greater than 100 mg/day/kg.

Products are for research use only. Not for human use.