Description
QX77 is a chaperone-mediated autophagy (CMA) activator and upregulates LAMP2A expression in vitro. QX77 induces Rab11 upregulation, rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 can impede self-renewal and promote differentiation of ES cells.
Product information
CAS Number: 1798331-92-6
Molecular Weight: 300.74
Formula: C16H13ClN2O2
Chemical Name: N-[4-(7-chloro-2H-1,4-benzoxazin-3-yl)phenyl]acetamide
Smiles: CC(=O)NC1C=CC(=CC=1)C1COC2=CC(Cl)=CC=C2N=1
InChiKey: PYCTUCLCTVWILY-UHFFFAOYSA-N
InChi: InChI=1S/C16H13ClN2O2/c1-10(20)18-13-5-2-11(3-6-13)15-9-21-16-8-12(17)4-7-14(16)19-15/h2-8H,9H2,1H3,(H,18,20)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : < 1 mg/mL (ultrasonic) (insoluble or slightly soluble).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
QX77 (48 hours) upregulates Rab11 expression levels in Ctns-/- MEFs. In Ctns-/- MEFs, QX77 recovers the down-regulated LAMP2A expression, and the Rab11-positive carrier vesicles recover the high-motility trafficking phenotype observe in wild-type cells. Treatment with CMA activator QX77 rescues Rab11 down-regulation and trafficking deficiency in cystinotic cells. QX77 treatment also increases LAMP2A localization at the lysosomal membrane. QX77 significantly increases the re-localization of LAMP2A at LAMP1-positive lysosomes in cystinotic cell, it corrects the localization of LAMP2A at the lysosomal membrane in cystinotic cells. QX77 protects cystinotic cells from the increased susceptibility to tert-butyl-hydroperoxide-induced oxidative stress and reconstitutes the resistant levels observed in wild-type cells. The effect of QX77 on cystinotic cell survival is dependent on LAMP2A expression. QX77 (10 μM; 0, 3 or 6 days) activates CMA and increases LAMP2A expression in D3 and E14 ES cell, it downregulates pluripotency factors and AP reactivity and partially lost the characteristic ES cell morphology.
Products are for research use only. Not for human use.
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