Description
DiFMUP is a fluorogenic substrate, and has been widely used for the continuous detection of phosphatase activities. DiFMUP is hydrolysis by a phosphatase results in the release of Xuorescent DIFMU, which can be easily followed in continuous mode by a Xuorescence reader.
Product information
CAS Number: 214491-43-7
Molecular Weight: 292.13
Formula: C10H7F2O6P
Chemical Name: [(6,8-difluoro-4-methyl-2-oxo-2H-chromen-7-yl)oxy]phosphonic acid
Smiles: CC1=CC(=O)OC2=C1C=C(F)C(OP(O)(O)=O)=C2F
InChiKey: DZANYXOTJVLAEE-UHFFFAOYSA-N
InChi: InChI=1S/C10H7F2O6P/c1-4-2-7(13)17-9-5(4)3-6(11)10(8(9)12)18-19(14,15)16/h2-3H,1H3,(H2,14,15,16)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
DIFMUP (100 μM; 0-4 min) is hydrolyzed by protein tyrosine phosphatase 1B (PTP1B) in a time-dependent manner and the initial rate of the reaction velocity is increased with the enzyme concentration (30-600 ng/mL).
Products are for research use only. Not for human use.
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