Description
BODIPY-cholesterol is an intrinsically lipophilic, and cell-permeable analog of cholesterol with a fluorescent BODIPY group. BODIPY-cholesterol can be used to monitor sterol uptake and inter-organelle sterol flux in cells. (Excitation/Emission: 480/508 nm).
Product information
CAS Number: 878557-19-8
Molecular Weight: 576.61
Formula: C36H51BF2N2O
Chemical Name: 8-{3-[(9aR,11aR)-7-hydroxy-9a,11a-dimethyl-1H,2H,3H,3aH,3bH,4H,6H,7H,8H,9H,9aH,9bH,10H,11H,11aH-cyclopenta[a]phenanthren-1-yl]butyl}-2,2-difluoro-4,6,10,12-tetramethyl-1λ⁵,3-diaza-2-boratricyclo[7.3.0.0³,⁷]dodeca-1(12),4,6,8,10-pentaen-1-ylium-2-uide
Smiles: CC(CCC1C2=C(C)C=C(C)N2[B-](F)(F)[N+]2C=1C(C)=CC=2C)C1CCC2C3CC=C4CC(O)CC[C@]4(C)C3CC[C@@]21C
InChiKey: CFFWELDLDWZNRO-SJIMSOPYSA-N
InChi: InChI=1S/C36H51BF2N2O/c1-21(8-10-29-33-22(2)18-24(4)40(33)37(38,39)41-25(5)19-23(3)34(29)41)30-12-13-31-28-11-9-26-20-27(42)14-16-35(26,6)32(28)15-17-36(30,31)7/h9,18-19,21,27-28,30-32,42H,8,10-17,20H2,1-7H3/t21?,27?,28?,30?,31?,32?,35-,36+/m0/s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
BODIPY-cholesterol assay To prepare a stock solution of BODIPY-cholesterol for this assay, the analogue is reconstituted in DMSO to a stock concentration of 1-10 μM. Cells diluted with PBS (or other buffers) are washed (300×g, 10 min) to remove excess diluent and the loose pellet resuspended with vehicle media to a proper concentration. Cell suspensions are labeled with BODIPY-cholesterol (final concentration of 0.5-5 µM), mixed thoroughly and incubated for 10 -20 mins at 37 °C. The cells are diluted with each of the capacitation media conditions as outlined in Incubation media to a final concentration and incubated for 2 h prior to flow cytometric assessment.
Products are for research use only. Not for human use.
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