Description
8-Azido-ATP, a photoreactable nucleotide analog, is useful for the identification of proteins, such as DNA-dependent RNA polymerase.
Product information
CAS Number: 53696-59-6
Molecular Weight: 548.19
Formula: C10H15N8O13P3
Chemical Name: ({[({[(2R,3S,4R,5R)-5-(6-amino-8-azido-9H-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy}(hydroxy)phosphoryl)oxy](hydroxy)phosphoryl}oxy)phosphonic acid
Smiles: [N-]=[N+]=NC1=NC2=C(N)N=CN=C2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O
InChiKey: PQISXOFEOCLOCT-UUOKFMHZSA-N
InChi: InChI=1S/C10H15N8O13P3/c11-7-4-8(14-2-13-7)18(10(15-4)16-17-12)9-6(20)5(19)3(29-9)1-28-33(24,25)31-34(26,27)30-32(21,22)23/h2-3,5-6,9,19-20H,1H2,(H,24,25)(H,26,27)(H2,11,13,14)(H2,21,22,23)/t3-,5-,6-,9-/m1/s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
8-Azido-ATP can be used to identify the viral RNA polymerase on the basis of the ability of the analog to inhibit transcription activity associated with rotavirus particles on exposure to UV light. 8-Azido-ATP (azido-ATP) decreases viral transcription in a dose-dependent manner. 8-Azido-ATP blocks Kir6.2ΔC26 currents rather less potently than ATP, half-maximal inhibition (Ki) occurring at 2.8 ± 0.4 mM (n = 6) compared with 172 ± 7 mM for ATP (n = 6). The Hill coefficients were 0.9 ± 0.2 for 8-azido-ATP and 1.3 ± 0.1 for ATP.
Products are for research use only. Not for human use.
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