Description
Acrylodan, reacted with thiols, is sensitive to the local environmental dipolarity and dynamics within the binding pocket surrounding Cys34.
Product information
CAS Number: 86636-92-2
Molecular Weight: 225.29
Formula: C15H15NO
Chemical Name: 1-[6-(dimethylamino)naphthalen-2-yl]prop-2-en-1-one
Smiles: CN(C)C1=CC2=CC=C(C=C2C=C1)C(=O)C=C
InChiKey: HMWAJFNEGAJETK-UHFFFAOYSA-N
InChi: InChI=1S/C15H15NO/c1-4-15(17)13-6-5-12-10-14(16(2)3)8-7-11(12)9-13/h4-10H,1H2,2-3H3
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 50 mg/mL (221.94 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Purification and Labeling of Act1: 1. 20-fold molar excess of acrylodan (Anaspec) was added to the polymerized actin and incubated on ice overnight. 2. The labeled actin is centrifuged (2 h, 4 °C, 100,000 × g) then the pellet was resuspended in 10 mm Tris, pH 7.5, 0.5 mm β-mercaptoethanol, 0.2 mm CaCl2, 50 μm ATP and dialyzed against the same buffer for 70 h at 4 °C. 3. The supernatant was collected and the actin concentration and degree of labeling were calculated using the extinction coefficient 26,600 M−1 cm−1 for actin at 290 nm, 18,500 M−1 cm−1 or acrylodan at 385 nm, and 71,000 M−1 cm−1 for Alexa Fluor 488 at 494 nm. 4. Acrylodan-labeled Act1 (AcrylAct1NAT) is dramatically different than that of EDTA-unfolded AcrylAct1 (AcrylAct1I3), which has a very similar emission spectrum to CCT-bound AcrylAct1.
Products are for research use only. Not for human use.
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