Description
Dihydrofluorescein diacetate is a fluorimetric probe mainly used for oxidative stress measurements, in both cell-free systems and cellular models.
Product information
CAS Number: 35340-49-9
Molecular Weight: 418.40
Formula: C24H18O7
Chemical Name: 2-[3,6-bis(acetyloxy)-9H-xanthen-9-yl]benzoic acid
Smiles: CC(=O)OC1C=C2OC3C=C(C=CC=3C(C3=CC=CC=C3C(O)=O)C2=CC=1)OC(C)=O
InChiKey: YKSJJXGQHSESKB-UHFFFAOYSA-N
InChi: InChI=1S/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : ≥ 106.5 mg/mL (254.54 mM).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Dihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizing species. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium. Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix. Dihydrofluorescein diacetate could be an useful and quantitative method for measuring the oxidative potential of nanoparticle-treated cells.
Products are for research use only. Not for human use.
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