Description
Calcein Blue, a membrane-impermeant fluorescent dye, is a coumarin derivative that contains an iminodiacetic acid structure. Calcein Blue is also a metallofluorochromic indicator.
Product information
CAS Number: 54375-47-2
Molecular Weight: 321.28
Formula: C15H15NO7
Chemical Name: 2-[(carboxymethyl)[(7-hydroxy-4-methyl-2-oxo-2H-chromen-8-yl)methyl]amino]acetic acid
Smiles: CC1=CC(=O)OC2=C1C=CC(O)=C2CN(CC(O)=O)CC(O)=O
InChiKey: RJPSHDMGSVVHFA-UHFFFAOYSA-N
InChi: InChI=1S/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 6 mg/mL (18.68 mM; Need ultrasonic and warming). H2O : 1 mg/mL (3.11 mM; ultrasonic and warming and heat to 80°C).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
The fluorescence of Calcein Blue (CB) is quenched by the Fe2+ ion in the Calcein Blue-Fe2+ complex. When dopamine is added to a solution of the Calcein Blue-Fe2+ complex, a dopamine-Fe2+ complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe2+ complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe2+ complex shows a high selectivity for dopamine among the catecholamines and related compounds examined.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.