Description
Fluorescein (Resorcinol phthalein) is a synthetic organic photoactive dye compound. Fluorescein is used as a fluorescent tracer in medicinal and biological applications and tumor infected tissues tracer.
Product information
CAS Number: 2321-07-5
Molecular Weight: 332.31
Formula: C20H12O5
Chemical Name: 3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one
Smiles: OC1=CC2OC3=CC(O)=CC=C3C3(OC(=O)C4=CC=CC=C34)C=2C=C1
InChiKey: GNBHRKFJIUUOQI-UHFFFAOYSA-N
InChi: InChI=1S/C20H12O5/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20/h1-10,21-22H
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 250 mg/mL (752.31 mM; Need ultrasonic). H2O : 1 mg/mL (3.01 mM; ultrasonic and warming and heat to 80°C).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Fluorescein is nearly inactive as a photodynamic sensitizer. Fluorescein consequently is almost useless as a photodynamic sensitizer, but it is of great value in fluorescence immunoassays. The fluorescein dye is a common fluorescent probe. Its very high molar absorptivity at the wavelength of the argon laser (488 nm), large fluorescence quantum yield and high photostability makes it a very useful and sensitive fluorescent label. Fluorescein is commercially available in many derivatives, such as fluorescein isothiocyanate and fluorescein succinimidyl ester, that can be covalently attached to macromolecules and to amino acids. The labeled molecules can be detected with very high sensitivity which is utilized in, for example, capillary electrophoresis. The emission spectrum of fluorescein overlaps extensively the absorption spectrum of tetramethyl rhodamine, which is a related strongly fluorescent dye, making this pair very suitable for energy transfer experiments to determine distances within and between labeled macromolecules. Fluorescein in aqueous solution occurs in cationic, neutral, anionic and dianionic forms making its absorption and fluoresence properties strongly pH dependent.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.