Description
CLEFMA is a curcuminoid with antitumor activity. CLEFMA inhibits tumor growth is associated with NF-κB-regulated anti-inflammatory and anti-metastatic effects.
Product information
CAS Number: 1246964-32-8
Molecular Weight: 442.29
Formula: C23H17Cl2NO4
Chemical Name: (2Z)-4-[(3E,5E)-3,5-bis[(2-chlorophenyl)methylidene]-4-oxopiperidin-1-yl]-4-oxobut-2-enoic acid
Smiles: OC(=O)/C=C\C(=O)N1C/C(=C\C2=CC=CC=C2Cl)/C(=O)/C(/C1)=C/C1=CC=CC=C1Cl
InChiKey: ABOUDPJRQGWQNW-GGDLZHBGSA-N
InChi: InChI=1S/C23H17Cl2NO4/c24-19-7-3-1-5-15(19)11-17-13-26(21(27)9-10-22(28)29)14-18(23(17)30)12-16-6-2-4-8-20(16)25/h1-12H,13-14H2,(H,28,29)/b10-9-,17-11+,18-12+
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
CLEFMA (1-100 μM; 24-72 h) has anti-proliferative activity. CLEFMA inhibits the viability of H441 and A549 cells, with IC50s of 6.4 and 8.9 μM, respectively. CLEFMA (5-10 μM; 24 h) induces apoptosis in H441 and A549 cells. CLEFMA (1 and 10 μM; 24 h) induces autophagic death in H441 cells. CLEFMA (1-20 μM) reduces the DNA-binding activity of NF-κB in H441 cells in a dose-dependent manner.
In Vivo:
CLEFMA (0.2-0.4 mg/kg; i.p. daily for 4 weeks) inhibits tumor growth and suppresses the uptake of FDG in tumor tissue in a xenograft mouse model. CLEFMA (0.2-0.4 mg/kg; i.p. daily for 4 weeks) down-regulates the expression of anti-apoptotic markers cIAP1, Bcl-xL, Bcl-2 and survivin, and induces the cleavage of pro-apoptotic protein BID and the expression of pro-apoptotic BAX in tumor tissue.
Products are for research use only. Not for human use.
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