Fura-2 AM

Fura-2 AM is a high affinity, intracellular, UV light-excitable and ratiometric fluorescent Ca2+ indicator.

Product information

CAS Number: 108964-32-5

Molecular Weight: 1001.85

Formula: C44H47N3O24

Chemical Name: (acetyloxy)methyl 2-({2-[(acetyloxy)methoxy]-2-oxoethyl}[2-(5-{[(acetyloxy)methoxy]carbonyl}-1,3-oxazol-2-yl)-5-(2-{2-[bis({2-[(acetyloxy)methoxy]-2-oxoethyl})amino]-5-methylphenoxy}ethoxy)-1-benzofuran-6-yl]amino)acetate

Smiles: CC1=CC(OCCOC2C=C3C=C(OC3=CC=2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C2=NC=C(O2)C(=O)OCOC(C)=O)=C(C=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O

InChiKey: VPSRLGDRGCKUTK-UHFFFAOYSA-N

InChi: InChI=1S/C44H47N3O24/c1-25-7-8-32(46(16-39(53)65-20-60-26(2)48)17-40(54)66-21-61-27(3)49)35(11-25)58-9-10-59-36-12-31-13-37(43-45-15-38(71-43)44(57)69-24-64-30(6)52)70-34(31)14-33(36)47(18-41(55)67-22-62-28(4)50)19-42(56)68-23-63-29(5)51/h7-8,11-15H,9-10,16-24H2,1-6H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). Fura-2 AM diffuses across the cell membrane and is de-esterified by cellular esterases to yield Fura-2 free acid. 1. First, prepare the 1 mM Fura-2 AM stock by adding 50 µL of DMSO to a 50 µg vial. It is important to use dry DMSO packed under nitrogen and it is necessary to remove the DMSO with a needle by puncturing the septum to prevent hydration of the DMSO. After preparing the Fura-2 AM solution keep it in a dark dry place. Fura-2 AM in DMSO is stable at RT for 24 hours and is stable at -20 degrees in a dry container for several months. 2. Aliquot 2 mL of culture media into a 15 mL conical tube, warm to 37 deg. and add 2 µL of Fura-2 AM stock to generate a 1µM Fura-2 AM solution. Vortex the solution vigorously for 1 min. 3. Transfer the loading solution to a 35 mm tissue culture dish and transfer the coverslip with the cells into the dish. 4. Incubate the neurons at 37 degrees for 30 minutes in a dark incubator. Time the incubation precisely. 5. Prepare a 35 mm dish containing 2 mL of tissue culture media without Fura-2 AM. Remove the coverslip from the loading solution and place in the new dish. 6. Mount the coverslip on the imaging chamber.

Products are for research use only. Not for human use.