Description
Rhod-2 AM is a fluorescent, mitochondrial probe (λex=552 nm, λem=581 nm).
Product information
CAS Number: 145037-81-6
Molecular Weight: 1123.94
Formula: C52H59BrN4O19
Chemical Name: 9-{4-[bis({2-[(acetyloxy)methoxy]-2-oxoethyl})amino]-3-(2-{2-[bis({2-[(acetyloxy)methoxy]-2-oxoethyl})amino]-5-methylphenoxy}ethoxy)phenyl}-6-(dimethylamino)-N,N-dimethyl-3H-xanthen-3-iminium bromide
Smiles: [Br-].CN(C)C1=CC2OC3=CC(C=CC3=C(C=2C=C1)C1=CC(OCCOC2=CC(C)=CC=C2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)=C(C=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)=[N+](C)C
InChiKey: RWXWZOWDWYQKBK-UHFFFAOYSA-M
InChi: InChI=1S/C52H59N4O19.BrH/c1-32-10-16-42(55(24-48(61)71-28-67-33(2)57)25-49(62)72-29-68-34(3)58)46(20-32)65-18-19-66-47-21-37(11-17-43(47)56(26-50(63)73-30-69-35(4)59)27-51(64)74-31-70-36(5)60)52-40-14-12-38(53(6)7)22-44(40)75-45-23-39(54(8)9)13-15-41(45)52;/h10-17,20-23H,18-19,24-31H2,1-9H3;1H/q+1;/p-1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
A high concentration of Ca2+ in mitochondria is illustrated by punctate labeling in cells, which is consistent with the location of Ca2+ in the mitochondria following fluorescence staining with Rhod-2 AM (Rhod2-AM) at 6 h p.i.. Inhibition of mitochondrial Ca2+ uptake by ruthenium red (RR) or 4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid (DIDS) in IMR5 cells infected with poliovirus (PV) is also illustrated following fluorescence staining with Rhod-2 AM at 6 h p.i..
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