Description
Cresyl Violet acetate is a red fluorescent stain, which can be used to stain neurons.
Product information
CAS Number: 10510-54-0
Molecular Weight: 321.33
Formula: C18H15N3O3
Chemical Name: 8-imino-8H-10-oxa-5-azatetraphen-12-amine; acetic acid
Smiles: CC(O)=O.NC1=CC2OC3=CC(=N)C=CC3=NC=2C2=CC=CC=C21
InChiKey: ORIKHDNZZCTIAD-UHFFFAOYSA-N
InChi: InChI=1S/C16H11N3O.C2H4O2/c17-9-5-6-13-14(7-9)20-15-8-12(18)10-3-1-2-4-11(10)16(15)19-13;1-2(3)4/h1-8,17H,18H2;1H3,(H,3,4)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 1 mg/mL (3.11 mM; Need ultrasonic). H2O : 0.67 mg/mL (2.09 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
The estimated total number of SG neurons is 27,485±3251 and 26,705±1823 in the PV and Cresyl Violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162±0.35 mm3 and 1.82±0.33 mm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63±951 μm3 and 3740.1±784 μm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68±50 μm3 and 126.51±33 μm3 in Cresyl Violet acetate staining and PV immunostaining sections, respectively.
Products are for research use only. Not for human use.
Payment & Security
Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.