Hematoxylin

Hematoxylin (Natural Black 1), a naturally occurring flavonoid compound derived from the logwood tree, Haematoxylon campechianum. Hematoxylin is a nuclear stain in histology and is also a potent Aβ42 fibrillogenesis inhibitor with an IC50 of 1.6 µM.

Product information

CAS Number: 517-28-2

Molecular Weight: 302.28

Formula: C16H14O6

Chemical Name: (1R,10S)-8-oxatetracyclo[8.7.0.0²,⁷.0¹²,¹⁷]heptadeca-2,4,6,12(17),13,15-hexaene-5,6,10,14,15-pentol

Smiles: OC1=CC2[C@@H]3C4=CC=C(O)C(O)=C4OC[C@]3(O)CC=2C=C1O

InChiKey: WZUVPPKBWHMQCE-XJKSGUPXSA-N

InChi: InChI=1S/C16H14O6/c17-10-2-1-8-13-9-4-12(19)11(18)3-7(9)5-16(13,21)6-22-15(8)14(10)20/h1-4,13,17-21H,5-6H2/t13-,16+/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 50 mg/mL (165.41 mM; Need ultrasonic). H2O : 6.67 mg/mL (22.07 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

When exposed to air, Hematoxylin is oxidized to reddish brown hematein. When oxidized to its hematein form and combined with a mordant, usually a metal salt, Hematoxylin stains tissue sections a deep blue to black color depending on the staining method. By itself, Hematoxylin is also amphoteric in its hematein form; it is red at acid pH and blue at alkaline pH. Differentiation following Hematoxylin staining removes nonspecific staining. Hematoxylin treatment greatly alleviates Aβ42-induced cytotoxicity in SH-SY5Y cells. Hematoxylin is a potential agent against Aβ fibrillogenesis and cytotoxicity. The Hematoxylin and Eosin (H&E) stained tissue section is the cornerstone of anatomical pathology diagnosis. The H&E procedure stains the nucleus and cytoplasm contrasting colors to readily differentiate cellular components.

In Vivo:

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). The method of H&E staining: 1. Place the glass slides that hold the paraffin sections in staining racks. Clear the paraffin from the samples in three changes of xylene for 2 min per change. 2. Hydrate the samples as follows. i. Transfer the slides through three changes of 100% ethanol for 2 min per change. ii. Transfer to 95% ethanol for 2 min. iii. Transfer to 70% ethanol for 2 min. iv. Rinse the slides in running tap water at room temperature for at least 2 min. 3. Stain the samples in Hematoxylin solution for 3 min. 4. Place the slides under running tap water at room temperature for at least 5 min. 5. Stain the samples in working eosin Y solution for 2 min. 6. Dehydrate the samples as follows. i. Dip the slides in 95% ethanol about 20 times. ii. Transfer to 95% ethanol for 2 min. iii. Transfer through two changes of 100% ethanol for 2 min per change. 7. Clear the samples in three changes of xylene for 2 min per change. 8. Place a drop of Permount over the tissue on each slide and add a coverslip. View the slides using a microscope.

Products are for research use only. Not for human use.