Description
DiI (DiIC18(3)) is a lipophilic carbocyanine fluorescent dye for membrane labeling. DiI has an absorption maximum at 549 nm and an emission maximum 565 nm. DiI is mildly fluorescent in aqueous suspension, but becomes bright when bound to cell membrane.
Product information
CAS Number: 41085-99-8
Molecular Weight: 933.87
Formula: C59H97ClN2O4
Chemical Name: 2-[3-(3,3-dimethyl-1-octadecyl-2,3-dihydro-1H-indol-2-ylidene)prop-1-en-1-yl]-3,3-dimethyl-1-octadecyl-3H-indol-1-ium perchlorate
Smiles: CC1(C)C(=CC=CC2=[N+](CCCCCCCCCCCCCCCCCC)C3=CC=CC=C3C2(C)C)N(CCCCCCCCCCCCCCCCCC)C2=CC=CC=C12.[O-]Cl(=O)(=O)=O
InChiKey: JVXZRNYCRFIEGV-UHFFFAOYSA-M
InChi: InChI=1S/C59H97N2.ClHO4/c1-7-9-11-13-15-17-19-21-23-25-27-29-31-33-35-41-50-60-54-46-39-37-44-52(54)58(3,4)56(60)48-43-49-57-59(5,6)53-45-38-40-47-55(53)61(57)51-42-36-34-32-30-28-26-24-22-20-18-16-14-12-10-8-2;2-1(3,4)5/h37-40,43-49H,7-36,41-42,50-51H2,1-6H3;(H,2,3,4,5)/q+1;/p-1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 12.5 mg/mL (13.39 mM; ultrasonic and warming and heat to 80°C).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Once bound to a membrane it diffuses laterally in 2 dimensions; if there is no diffusion barrier, DiI generally stains the whole leaflet (one surface) of a biological membrane rapidly, but does not readily flip across to the other leaflet.
In Vivo:
DiI-labeled motoneurons have remained viable for up to 4 weeks in culture and up to one year in vivo
Products are for research use only. Not for human use.
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