Description
JF635, SE (JF635, NHS) is a red fluorogenic fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. JF635, SE can be used for live cell imaging.
Product information
Molecular Weight: 629.68
Formula: C33H29F2N3O6Si
Chemical Name: 1-[9-(2-carboxylato-5-{[(2,5-dioxopyrrolidin-1-yl)oxy]carbonyl}phenyl)-13-(3-fluoroazetidin-1-yl)-2,2-dimethyl-2-silatricyclo[8.4.0.0³,⁸]tetradeca-1(14),3,6,8,10,12-hexaen-5-ylidene]-3-fluoro-1λ⁵-azetidin-1-ylium
Smiles: C[Si]1(C)C2=CC(C=CC2=C(C2=CC=C(C=C12)N1CC(F)C1)C1=CC(=CC=C1C([O-])=O)C(=O)ON1C(=O)CCC1=O)=[N+]1CC(F)C1
InChiKey: YVKNNZJUJGADIJ-UHFFFAOYSA-N
InChi: InChI=1S/C33H29F2N3O6Si/c1-45(2)27-12-21(36-14-19(34)15-36)4-7-24(27)31(25-8-5-22(13-28(25)45)37-16-20(35)17-37)26-11-18(3-6-23(26)32(41)42)33(43)44-38-29(39)9-10-30(38)40/h3-8,11-13,19-20H,9-10,14-17H2,1-2H3
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Maximum absorption wavelength (λabs)=635 nm, maximum emission wavelength (λem)=652 nm. JF635 can serve as a ligand for self-labeling tag (such as HaloTag and SNAP-tag). The SNAP-tag ligand of JF635 (29) effectively labels SNAP-tag fusions in cells and the JF635–HaloTag ligand (27) could be used in a two-color experiment with JF525-SNAP-tag ligand,
Products are for research use only. Not for human use.
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