Description
RapiFluor-MS can be used a marker for LC-MS/MS analysis of N-glycans, which provides the highest MS signal enhancement for neutral glycans.
Product information
CAS Number: 1429047-69-7
Molecular Weight: 427.45
Formula: C21H25N5O5
Chemical Name: 2,5-dioxopyrrolidin-1-yl N-(2-{[2-(diethylamino)ethyl]carbamoyl}quinolin-6-yl)carbamate
Smiles: CCN(CCNC(=O)C1=CC=C2C=C(C=CC2=N1)NC(=O)ON1C(=O)CCC1=O)CC
InChiKey: BWBDAEIIXBEFSS-UHFFFAOYSA-N
InChi: InChI=1S/C21H25N5O5/c1-3-25(4-2)12-11-22-20(29)17-7-5-14-13-15(6-8-16(14)24-17)23-21(30)31-26-18(27)9-10-19(26)28/h5-8,13H,3-4,9-12H2,1-2H3,(H,22,29)(H,23,30)
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 12.5 mg/mL (29.24 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Sample preparation: (1) Denaturation of glycoprotein; (2) Deglycosylation using PNGase F; (3) N-glycans labeling with RapiFluor-MS reagent for enhanced fluorescence and MS signal; Dissolve 9 mg of RapiFluor-MS reagent in 110 μL of anhydrous DMF. Aspirate and dispense the solution or vortex to mix. Add 10 μL of RapiFluor-MS solution to the deglycosylated mixture in the 1 mL tube. Aspirate and dispense to mix. (4) Purification using a SPE μElution plate in HILIC mode.
Products are for research use only. Not for human use.
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