1-methyl-D-tryptophan (D-1MT) --- IDO Inhibitor

1-methyl-D-tryptophan (D-1MT) is a potent and selective indoleamine 2, 3-dioxygenase (IDO) inhibitor with IC50 ~7 μM. It disrupts tryptophan catabolism and enhances the antitumor and antiviral immunoresponses of CD8+ T-cells in vitro. It can reduce tumor volume in mice with xenografts overexpressing IDO.

Product information

CAS Number: 110117-83-4

Molecular Weight: 218.25

Formula: C12H14N2O2

Synonym:

D-1MT

1-MT

1-Methyl-D-tryptophan

Indoximod

(2R)-2-amino-3-(1-methylindol-3-yl)propanoic acid

Related CAS Number:

110117-83-4

Chemical Name: 1-methyl-D-tryptophan

Smiles: CN1C=C(C[C@@H](N)C(O)=O)C2=CC=CC=C12

InChiKey: ZADWXFSZEAPBJS-SNVBAGLBSA-N

InChi: InChI=1S/C12H14N2O2/c1-14-7-8(6-10(13)12(15)16)9-4-2-3-5-11(9)14/h2-5,7,10H,6,13H2,1H3,(H,15,16)/t10-/m1/s1

Technical Data

Appearance: White to off-white solid powder

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: H2O : 5 mg/mL (22.91 mM; ultrasonic and adjust pH to 2 with HCl), 5 mM (warming); DMSO : 0.55 mg/mL (2.52 mM; Need ultrasonic and warming).

Shipping Condition: Room temperature in continental US; may vary elsewhere.

Storage Condition: Powder -20°C for 3 years, 4°C for 1 year;

Shelf Life: ≥36 months if stored properly.

Stock Solution Storage: DMSO: 4^oC for 3 months.

Drug Formulation: To be determined.

HS Tariff Code: 2934.99.9001

How to use

In Vitro:

D-1MT was used at 10-50 µM final concentration in various in vitro assays.

In Vivo:

D-1MT was dosed at 5 mg/Kg orally to mice bearing A20 B-cell lymphoma.

References:

1. Rytelewski M, et al. Suppression of immunodominant antitumor and antiviral CD8+ T cell responses by indoleamine 2,3-dioxygenase. (2014) PLoS One. 9(2):e90439.
2. Nakamura N, et al. Effects of indoleamine 2,3-dioxygenase inhibitor in non-Hodgkin lymphoma model mice. (2015) Int J Hematol. 102(3):327-34.

Products are for research use only. Not for human use.

Documents