A77-01

A77-01 is an inhibitor of TGF-β type I receptor superfamily activin-like kinase ALK5 with an IC50 of 25nM.

Product information

CAS Number: 607737-87-1

Molecular Weight: 286.33

Formula: C18H14N4

Chemical Name: 4-(5-(6-methylpyridin-2-yl)-1H-pyrazol-4-yl)quinoline

Smiles: CC1=CC=CC(=N1)C1NN=CC=1C1=CC=NC2=CC=CC=C21

InChiKey: KJTYZDORHCDZPS-UHFFFAOYSA-N

InChi: InChI=1S/C18H14N4/c1-12-5-4-8-17(21-12)18-15(11-20-22-18)13-9-10-19-16-7-3-2-6-14(13)16/h2-11H,1H3,(H,20,22)

Technical Data

Appearance: Solid Power.

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO, not in water

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined.

HS Tariff Code: 382200

How to use

In Vitro:

A77-01 (0.01-10 µM; 25 hours; Mv1Lu cells) treatment effectively inhibits TGF-β-induced transcriptional activation in concentration-dependent fashion. A77-01 (1 µM; 24-72 hours; Mv1Lu cells) treatment efficiently prevents the growth-inhibitory effects of TGF-β. A77-01 (1 µM; 30 minutes; HaCaT cells) treatment inhibits TGF-β-induced phosphorylation of Smad2.

References:

1. Tojo M, et al. The ALK-5 inhibitor A-83-01 inhibits Smad signaling and epithelial-to-mesenchymal transition by transforming growth factor-beta. Cancer Sci. 2005 Nov;96(11):791-800.

Products are for research use only. Not for human use.