Puromycin aminonucleoside

Puromycin aminonucleoside (NSC 3056, PAN, Stylomycin aminonucleoside, ARDMA, SAN), the aminonucleoside portion of the antibiotic puromycin, is a reversible inhibitor of dipeptidyl-peptidase II and cytosol alanyl aminopeptidase that induces apoptosis in mesangial cells (MCs) accompanied by declined cell viability and enhanced inflammatory response.

Product information

CAS Number: 58-60-6

Molecular Weight: 294.31

Formula: C12H18N6O3

Synonym:

ARDMA

NSC 3056

SAN

Stylomycin aminonucleoside

Chemical Name: (2R,3R,4S,5S)-4-amino-2-[6-(dimethylamino)-9H-purin-9-yl]-5-(hydroxymethyl)oxolan-3-ol

Smiles: CN(C)C1=NC=NC2=C1N=CN2[C@@H]1O[C@H](CO)[C@@H](N)[C@H]1O

InChiKey: RYSMHWILUNYBFW-GRIPGOBMSA-N

InChi: InChI=1S/C12H18N6O3/c1-17(2)10-8-11(15-4-14-10)18(5-16-8)12-9(20)7(13)6(3-19)21-12/h4-7,9,12,19-20H,3,13H2,1-2H3/t6-,7-,9-,12-/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO: 59 mg/mL(200.46 mM). Water: 30 mg/mL(101.93 mM).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Puromycin aminonucleoside (PAN) induces MC apoptosis accompanied by the declined cell viability and enhanced inflammatory response. In PAN-treated MCs, ERRα overexpression further aggravates PAN-induced apoptosis.

In Vivo:

In agreement with the in vitro study, increased ERRα expression is observed in line with enhanced apoptotic response in renal cortex from PAN-treated rats.

References:

1. Wei Gong, et al. Am J Physiol Renal Physiol. 2019 May 1;316(5):F906-F913.

Products are for research use only. Not for human use.