Nitro-PDS-Tubulysin M

Nitro-PDS-Tubulysin M is a drug-linker conjugate for ADC with potent antitumor activity by using Tubulysin M (a tubulin polymerization inhibitor), linked via the ADC linker Nitro-PDS.

Product information

CAS Number: 1941168-69-9

Molecular Weight: 1106.40

Formula: C54H73N8O11S3

Chemical Name: (2R)-2-{[(1S,2S)-1-{[(1R,3R)-1-(acetyloxy)-1-(4-{[(2R,4S)-4-carboxy-4-methyl-1-phenylbutan-2-yl]carbamoyl}-1,3-thiazol-2-yl)-4-methylpentan-3-yl](methyl)carbamoyl}-2-methylbutyl]carbamoyl}-1-methyl-1-{[4-({[(2R)-2-[(6-nitropyridin-3-yl)disulfanyl]propoxy]carbonyl}amino)phenyl]methyl}piperidin-1-ium

Smiles: CC(=O)O[C@H](C[C@H](C(C)C)N(C)C(=O)[C@@H](NC(=O)[C@H]1CCCC[N+]1(C)CC1C=CC(=CC=1)NC(=O)OC[C@@H](C)SSC1C=NC(=CC=1)[N+]([O-])=O)[C@@H](C)CC)C1=NC(=CS1)C(=O)N[C@H](C[C@H](C)C(O)=O)CC1C=CC=CC=1

InChiKey: FVZABGIKJSSIQM-KDOZOHQXSA-O

InChi: InChI=1S/C54H72N8O11S3/c1-10-34(4)48(52(66)60(8)44(33(2)3)28-46(73-37(7)63)51-58-43(32-74-51)49(64)56-41(26-35(5)53(67)68)27-38-16-12-11-13-17-38)59-50(65)45-18-14-15-25-62(45,9)30-39-19-21-40(22-20-39)57-54(69)72-31-36(6)75-76-42-23-24-47(55-29-42)61(70)71/h11-13,16-17,19-24,29,32-36,41,44-46,48H,10,14-15,18,25-28,30-31H2,1-9H3,(H3-,56,57,59,64,65,67,68,69)/p+1/t34-,35-,36+,41+,44+,45+,46+,48-,62?/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

ADC2-2 (anti-CD22 10F4v3 LC K149C Nitro-PDS-Tubulysin M) displays target-specific killing in WSU-DLCL2 human diffuse large B-cell lymphoma tumors with IC50 of 0.147 nM, and ADC2-1 (anti-Napi2b 10H1.11.4B LC K149C Nitro-PDS-Tubulysin M) displays target-specific killing in human ovarian cancer IGROV-1 and OVCAR-3x2.1 with IC50s of 2.07 nM, 1.004 nM, respectively.

References:

1. FLYGARE, John A, et al. Quaternary amine compounds and antibody-drug conjugates thereof. WO2016090050A1.
2. Staben LR, et al. Targeted drug delivery through the traceless release of tertiary and heteroaryl amines from antibody-drug conjugates. Nat Chem. 2016 Dec;8(12):1112-1119.

Products are for research use only. Not for human use.