TD-428

TD-428 is a highly specific BRD4 degrader with a DC50 of 0.32 nM. TD-428 is a BET PROTAC, which comprises TD-106 (a CRBN ligand) linked to JQ1 (a BET inhibitor). TD-428 efficiently induce BET protein degradation.

Product information

CAS Number: 2334525-50-5

Molecular Weight: 879.38

Formula: C43H43ClN10O7S

Chemical Name: 2-((S)-4-(4-chlorophenyl)-2, 3, 9-trimethyl-6H-thieno[3, 2-f][1, 2, 4]triazolo[4, 3-a][1, 4]diazepin-6-yl)-N-(3-(2-(2-(2-((3-(2, 6-dioxopiperidin-3-yl)-4-oxo-3, 4-dihydrobenzo[d][1, 2, 3]triazin-5-yl)amino)ethoxy)ethoxy)ethoxy)phenyl)acetamide

Smiles: CC1=NN=C2[C@H](CC(=O)NC3=CC(=CC=C3)OCCOCCOCCNC3=CC=CC4N=NN(C5CCC(=O)NC5=O)C(=O)C=43)N=C(C3=CC=C(Cl)C=C3)C3=C(SC(C)=C3C)N12

InChiKey: XEZUBGASURUNMF-CDRRMRQFSA-N

InChi: InChI=1S/C43H43ClN10O7S/c1-24-25(2)62-43-37(24)39(27-10-12-28(44)13-11-27)47-33(40-51-49-26(3)53(40)43)23-36(56)46-29-6-4-7-30(22-29)61-21-20-60-19-18-59-17-16-45-31-8-5-9-32-38(31)42(58)54(52-50-32)34-14-15-35(55)48-41(34)57/h4-13,22,33-34,45H,14-21,23H2,1-3H3,(H,46,56)(H,48,55,57)/t33-,34?/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Soluble in DMSO

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

TD-428, which comprises TD-106 linked to a BET inhibitor, JQ1 efficiently induce BET protein degradation in the prostate cancer cell line 22Rv1. TD-428 (0.01-10, 000 nM; 72 hours) inhibits the proliferation of 22RV1 cells with a CC50 of  20.1 nM. TD-428 (1 nM-10 μM; 12 hours) induces the degradation of BRD4, IKZF1, and IKZF3 proteins.

References:

1. Scheepstra M, et al. Bivalent Ligands for Protein Degradation in Drug Discovery. Comput Struct Biotechnol J. 2019 Jan 25;17:160-176.

Products are for research use only. Not for human use.