PKI(5-24)

PKI(5-24) is a potent, competitive, and synthetic peptide inhibitor of PKA (cAMP-dependent protein kinase), with a Ki of 2.3 nM. PKI(5-24) corresponds to residues 5-24 in the naturally occurring heat-stable protein kinase inhibitor.

Product information

CAS Number: 99534-03-9

Molecular Weight: 2222.38

Formula: C94H148N32O31

Synonym:

Thr-Thr-Tyr-Ala-Asp-Phe-Ile-Ala-Ser-Gly-Arg-Thr-Gly-Arg-Arg-Asn-Ala-Ile-His-Asp

Chemical Name: (2S, 5S, 8S, 11S, 14S, 17S, 20S, 26S, 29S, 35S, 38S, 41S, 44S, 47S)-5-((1H-imidazol-4-yl)methyl)-14-(2-amino-2-oxoethyl)-47-((S)-2-((S)-2-((2S, 3R)-2-((2S, 3R)-2-amino-3-hydroxybutanamido)-3-hydroxybutanamido)-3-(4-hydroxyphenyl)propanamido)propanamido)-44-benzyl-8, 41-di-sec-butyl-17, 20, 29-tris(3-guanidinopropyl)-26-((R)-1-hydroxyethyl)-35-(hydroxymethyl)-11, 38-dimethyl-4, 7, 10, 13, 16, 19, 22, 25, 28, 31, 34, 37, 40, 43, 46-pentadecaoxo-3, 6, 9, 12, 15, 18, 21, 24, 27, 30, 33, 36, 39, 42, 45-pentadecaazaoctatetracontane-1, 2, 48-tricarboxylic acid

Smiles: C[C@@H](O)[C@H](NC(=O)[C@@H](N)[C@@H](C)O)C(=O)N[C@@H](CC1C=CC(O)=CC=1)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1C=CC=CC=1)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)NCC(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](C)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CC(O)=O)C(O)=O

InChiKey: AXOXZJJMUVSZQY-OCDBTFLZSA-N

InChi: InChI=1S/C94H148N32O31/c1-11-42(3)70(124-85(150)58(31-50-19-14-13-15-20-50)117-84(149)61(35-67(135)136)116-74(139)44(5)110-81(146)57(32-51-24-26-53(131)27-25-51)119-90(155)73(49(10)130)126-86(151)69(96)47(8)128)88(153)112-45(6)75(140)122-63(40-127)77(142)107-38-65(133)114-55(22-17-29-105-93(99)100)80(145)125-72(48(9)129)87(152)108-39-66(134)113-54(21-16-28-104-92(97)98)78(143)115-56(23-18-30-106-94(101)102)79(144)118-60(34-64(95)132)82(147)111-46(7)76(141)123-71(43(4)12-2)89(154)120-59(33-52-37-103-41-109-52)83(148)121-62(91(156)157)36-68(137)138/h13-15,19-20,24-27,37,41-49,54-63,69-73,127-131H,11-12,16-18,21-23,28-36,38-40,96H2,1-10H3,(H2,95,132)(H,103,109)(H,107,142)(H,108,152)(H,110,146)(H,111,147)(H,112,153)(H,113,134)(H,114,133)(H,115,143)(H,116,139)(H,117,149)(H,118,144)(H,119,155)(H,120,154)(H,121,148)(H,122,140)(H,123,141)(H,124,150)(H,125,145)(H,126,151)(H,135,136)(H,137,138)(H,156,157)(H4,97,98,104)(H4,99,100,105)(H4,101,102,106)/t42-,43-,44-,45-,46-,47+,48+,49+,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,69-,70-,71-,72-,73-/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: H2O : 33.33 mg/mL (15.00 mM; Need ultrasonic)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

References:

1. Cheng HC, et al. A potent synthetic peptide inhibitor of the cAMP-dependent protein kinase. J Biol Chem. 1986;261(3):989-992.
2. Ruth P, et al F. A cGMP kinase mutant with increased sensitivity to the protein kinase inhibitor peptide PKI(5-24). Biol Chem. 1996;377(7-8):513-520.

Products are for research use only. Not for human use.