Ganoderic acid D

Ganoderic acid D, a highly oxygenated tetracyclic triterpenoid, is the major active component of Ganoderma lucidum. Ganoderic acid D upregulates the protein expression of SIRT3 and induces the deacetylated cyclophilin D (CypD) by SIRT3. Ganoderic acid D inhibits the energy reprogramming of colon cancer cells including glucose uptake, lactate production, pyruvate and acetyl-coenzyme production in colon cancer cells. Ganoderic acid D induces HeLa human cervical carcinoma apoptosis.

Product information

CAS Number: 108340-60-9

Molecular Weight: 514.65

Formula: C30H42O7

Chemical Name: (6R)-6-[(1R, 3aR, 4S, 5aR, 9aS, 11aR)-4-hydroxy-3a, 6, 6, 9a, 11a-pentamethyl-3, 7, 10-trioxo-1H, 2H, 3H, 3aH, 4H, 5H, 5aH, 6H, 7H, 8H, 9H, 9aH, 10H, 11H, 11aH-cyclopenta[a]phenanthren-1-yl]-2-methyl-4-oxoheptanoic acid

Smiles: CC1(C)[C@@H]2C[C@H](O)C3=C(C(=O)C[C@]4(C)[C@H](CC(=O)[C@]43C)[C@H](C)CC(=O)CC(C)C(O)=O)[C@@]2(C)CCC1=O

InChiKey: YTVGSCZIHGRVAV-IYAQLQCNSA-N

InChi: InChI=1S/C30H42O7/c1-15(10-17(31)11-16(2)26(36)37)18-12-23(35)30(7)25-19(32)13-21-27(3,4)22(34)8-9-28(21,5)24(25)20(33)14-29(18,30)6/h15-16,18-19,21,32H,8-14H2,1-7H3,(H,36,37)/t15-,16?,18-,19+,21+,28+,29-,30+/m1/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 100 mg/mL (194.31 mM; Need ultrasonic) H2O : < 0.1 mg/mL (insoluble)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥360 days if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Ganoderic acid D can inhibit the growth of numerous cancer cell lines and it inhibits HeLa human cervical carcinoma cells with an IC50 of 17.3 mM. Ganoderic acid D (1-50 μM; 24-72 hours) reduces the cell survival rate in a dose- and time-dependent manner. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces G2/M phase arrest. Ganoderic acid D (10, 50 μM; 24, 48 hours) induces a morphological change typical of apoptosis in HeLa cells. Ganoderic acid D (10 μM; 48 hours) up-regulates 14-3-3E and PRDX3.

References:

1. Liu Z, et al. Effect of ganoderic acid D on colon cancer Warburg effect: Role of SIRT3/cyclophilin D. Eur J Pharmacol. 2018 Apr 5;824:72-77.
2. Yue QX, et al. Proteomics characterization of the cytotoxicity mechanism of ganoderic acid D and computer-automated estimation of the possible drug target network. Mol Cell Proteomics. 2008 May;7(5):949-61.

Products are for research use only. Not for human use.