Compound 48/80 trihydrochloride

Compound 48/80 trihydrochloride (C48/80 trihydrochloride) is a mixture of condensation products of N-methyl-p-methoxyphenethylamine with formaldehyde. Compound 48/80 trihydrochloride is also a histamine releaser and a mast cell degranulator. Compound 48/80 inhibits phosphatidylinositol-specific phospholipase C activity from human platelets .

Product information

CAS Number: 848035-21-2

Molecular Weight: 629.10

Formula: C32H48Cl3N3O3

Synonym:

C48/80 trihydrochloride

Chemical Name: tris(chlorohydrogen); {2-[4-methoxy-3,5-bis({2-methoxy-5-[2-(methylamino)ethyl]phenyl}methyl)phenyl]ethyl}(methyl)amine

Smiles: Cl.Cl.Cl.COC1C(CC2=CC(CCNC)=CC=C2OC)=CC(CCNC)=CC=1CC1=CC(CCNC)=CC=C1OC

InChiKey: KIAWAQJORBNOIF-UHFFFAOYSA-N

InChi: InChI=1S/C32H45N3O3.3ClH/c1-33-14-11-23-7-9-30(36-4)26(17-23)21-28-19-25(13-16-35-3)20-29(32(28)38-6)22-27-18-24(12-15-34-2)8-10-31(27)37-5;;;/h7-10,17-20,33-35H,11-16,21-22H2,1-6H3;3*1H

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 25 mg/mL (39.74 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Compound 48/80 trihydrochloride (C48/80 trihydrochloride) inhibits both cytosolic and particulate phosphatidylinositol-specific phospholipase C activities with a similar efficiency; IC50 values are 2.1 μg/ml (supernatant) and 5.0 μg /ml (particulate fraction). The aggregation of human platelets induced by ADP and PAF-acether is inhibited by Compound 48/80.

In Vivo:

Compound 48/80 trihydrochloride (0.75 mg/kg; i.p.; Killed after 0.5, 3 or 6 h) increases serum serotonin, histamine and corticosterone levels at 0.5 h, but their increases were reduced thereafter. Compound 48/80 trihydrochloride causes oxidative stress in rat adrenal gland through mast cell degranulation.

References:

1. Bronner C, et al. Compound 48/80 is a potent inhibitor of phospholipase C and a dual modulator of phospholipase A2 from human platelet. Biochim Biophys Acta. 1987 Aug 15;920(3):301-5.
2. Schemann M, et al. The mast cell degranulator compound 48/80 directly activates neurons. PLoS One. 2012;7(12):e52104.
3. Kaida S, et al. Compound 48/80 causes oxidative stress in the adrenal gland of rats through mast cell degranulation. Free Radic Res. 2010 Feb;44(2):171-80.

Products are for research use only. Not for human use.