Scoparone

Scoparone (6,7-dimethoxycoumarin, Aesculetin dimethyl ether, 6,7-Dimethylesculetin, Escoparone) is a natural organic compound discovered from Chinese herb Artemisia scoparia with anticoagulant, hypolipidemic, vasorelaxant, antioxidant, and anti-inflammatory actions.

Product information

CAS Number: 120-08-1

Molecular Weight: 206.19

Formula: C11H10O4

Chemical Name: 6,7-dimethoxy-2H-chromen-2-one

Smiles: COC1C=C2OC(=O)C=CC2=CC=1OC

InChiKey: GUAFOGOEJLSQBT-UHFFFAOYSA-N

InChi: InChI=1S/C11H10O4/c1-13-9-5-7-3-4-11(12)15-8(7)6-10(9)14-2/h3-6H,1-2H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: Solubility (25°C). 41 mg/mL(198.84 mM). Insoluble.

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

The proliferation of VSMCs is significantly attenuated by scoparone in a dose-dependent manner. Scoparone markedly reduces the serum-stimulated accumulation of cells in the S phase and concomitantly increases the proportion of cells in the G0/G1 phase, which is consistent with the reduced expression of cyclin D1, phosphorylated Rb and survivin in the VSMCs. Cell adhesion markers, such as MCP-1 and ICAM-1, are significantly reduced by scoparone. It attenuates the increase in cyclin D promoter activity by inhibiting the activities of both the WT and active forms of STAT3. scoparone blocks the accumulation of STAT3 transported from the cytosol to the nucleus, leading to the suppression of VSMC proliferation through G1 phase arrest and the inhibition of Rb phosphorylation. This activity occurs independent of the form of STAT3 and upstream of kinases, such as Jak and Src, which are correlated with abnormal vascular remodeling due to the presence of an excess of growth factors following vascular injury. The protective effect of scoparone involves anti-inflammatory functions that occur via the inhibition of NF-κB activity, leading to the downregulation of pro-inflammatory cytokines and chemokines, such as tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-8 and monocyte chemoattractant protein (MCP)-1.

In Vivo:

The free radical scavenging property of scoparone leads to the attenuation of intima thickening and improves hemodynamics in cholesterol-fed alloxan-diabetic rabbits concurrent with decreases in plasma lipid and lipoprotein cholesterol concentrations. Scoparone exhibited therapeutic efficacies on liver damage in vivo.

References:

1. Xu M, et al. Scoparone Protects Against Pancreatic Fibrosis via TGF-β/Smad Signaling in Rats. Cell Physiol Biochem. 2016;40(1-2):277-286. Epub 2016 Nov 18.

Products are for research use only. Not for human use.