Cytochalasin J

Cytochalasin J can alter mitotic spindle microtubule organization and kinetochore structure. The cytochalasins are cell-permeable fungal metabolites inhibiting actin polymerization, which can alter diverse processes including cell movement, growth, phagocytosis, degranulation, and secretion. In vitro: Cytochalasin J was identified as a deacetylated analog of cytochalasin H that weakly inhibited actin assembly but significantly altered mitotic spindle microtubule organization and kinetochore structure . In a previous study, cytochalasin J treatment of prometaphase cells reduced the number of kMTs and the size and organization of the kinetochore lamina. Moreover, in approximately 30% of cells treated with cytochalasin J, the failure of a small number of chromosomes to attach to spindle fibers could be documented. These chromosomes showed a significant change in the organization of the kinetochore laminae. Light microscopic analyses of cells treated with cytochalasin J revealed loss of chromosome congression, with chromsomes usually located at the periphery of the spindle. Cells treated with 10 microg/ml cytochalasin J for 10 min and released into tissue culture medium showed a resumption of chromosome motion within a few minutes, both during congression and anaphase . In vivo: Up to now, there is no animal in vivo data reported. Clinical trial: So far, no clinical study has been conducted.

Product information

CAS Number: 53760-20-6

Molecular Weight: 451.60

Formula: C28H37NO4

Chemical Name: (3S,3aR,4S,6S,6aR,10S,12R,15R,15aR)-3-benzyl-6,12,15-trihydroxy-4,10,12-trimethyl-5-methylidene-1H,2H,3H,3aH,4H,5H,6H,6aH,9H,10H,11H,12H,15H-cycloundeca[e]isoindol-1-one

Smiles: C[C@@]1(O)C[C@@H](C)CC=C[C@H]2[C@H](O)C(=C)[C@@H](C)[C@H]3[C@H](CC4C=CC=CC=4)NC(=O)[C@@]23[C@H](O)C=C1 |t:7,34|

InChiKey: UKQNIEMKORIOQM-RVVPKKHWSA-N

InChi: InChI=1S/C28H37NO4/c1-17-9-8-12-21-25(31)19(3)18(2)24-22(15-20-10-6-5-7-11-20)29-26(32)28(21,24)23(30)13-14-27(4,33)16-17/h5-8,10-14,17-18,21-25,30-31,33H,3,9,15-16H2,1-2,4H3,(H,29,32)/b12-8-,14-13-/t17-,18+,21-,22-,23+,24-,25+,27-,28+/m0/s1

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

Products are for research use only. Not for human use.