PPACK Dihydrochloride

PPACK Dihydrochloride is the dihydrochloride form of its active component PPACK (D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone), a potent, selective and irreversible inhibitor of thrombin that inhibits human α-thrombin with inhibition constant value of 0.24 nM. PPACK covalently binds to the specific active-site Ser of thrombin and cross links with His57 at the binding site forming a tetrahedral PPACK-thrombin complex. The complex further binds to and saturates the high-affinity functional thrombin receptors eventually leading to the inhibition of thrombin activation. PPACK is currently being investigated as an anti-coagulant to prevent blood coagulation. Study results show that PPACK is able to dose-dependently inhibit thrombin-induced platelet accumulation. .Jun T. Liu, William Paul, Michael Emerson, Carla Cicala and Clive P. Page. Thrombin inhibitors and anti-coagulants on thrombin-induced embolization in rabbit cranial vasculature. European Journal of Pharmacology 264 (1994) 183-190 .Ildiko M. Kovach, Paul Kelley, Carol Eddy, Frank Jordan and Ahmet Baykal. Proton bridging in the interactions of thrombin with small inhibitors. Biochemistry. 2009; 48(30): 7296-7304 .Martha E. Lyon, James S. Fine, Paul J. Henderson and Andrew W. Lyon. D-Phenylalanyl-L-prolyl-L-arginine chloromethyl ketone (PPACK): alternative anticoagulant to heparin salts for blood gas and electrolyte specimens. CLIN. CHEM. 41/7, 1038-1041 (1995)

Product information

Molecular Weight: 450.96

Formula: C21H31ClN6O3

Chemical Name: 2-amino-N-(1-{1-chloro-6-[(diaminomethylidene)amino]-2-oxohexan-3-yl}pyrrolidine-2-carbonyl)-3-phenylpropanamide

Smiles: NC(N)=NCCCC(C(=O)CCl)N1CCCC1C(=O)NC(=O)C(N)CC1C=CC=CC=1

InChiKey: BBLOJOHIPXAIRZ-UHFFFAOYSA-N

InChi: InChI=1S/C21H31ClN6O3/c22-13-18(29)16(8-4-10-26-21(24)25)28-11-5-9-17(28)20(31)27-19(30)15(23)12-14-6-2-1-3-7-14/h1-3,6-7,15-17H,4-5,8-13,23H2,(H4,24,25,26)(H,27,30,31)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

Products are for research use only. Not for human use.