Description
Fluo-4 AM is a cell-permeable Ca2+ indicator.
Product information
CAS Number: 273221-67-3
Molecular Weight: 1096.94
Formula: C51H50F2N2O23
Chemical Name: (acetyloxy)methyl 2-({2-[2-(5-{6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl}-2-[bis({2-[(acetyloxy)methoxy]-2-oxoethyl})amino]phenoxy)ethoxy]-4-methylphenyl}({2-[(acetyloxy)methoxy]-2-oxoethyl})amino)acetate
Smiles: CC1=CC(OCCOC2=CC(=CC=C2N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O)C2C3=CC(F)=C(C=C3OC3=CC(=O)C(F)=CC3=2)OCOC(C)=O)=C(C=C1)N(CC(=O)OCOC(C)=O)CC(=O)OCOC(C)=O
InChiKey: QOMNQGZXFYNBNG-UHFFFAOYSA-N
InChi: InChI=1S/C51H50F2N2O23/c1-28-7-9-39(54(19-47(62)74-24-69-30(3)57)20-48(63)75-25-70-31(4)58)45(13-28)66-11-12-67-46-14-34(8-10-40(46)55(21-49(64)76-26-71-32(5)59)22-50(65)77-27-72-33(6)60)51-35-15-37(52)41(61)17-42(35)78-43-18-44(38(53)16-36(43)51)73-23-68-29(2)56/h7-10,13-18H,11-12,19-27H2,1-6H3
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
Fluo-4 AM is a fluorescent dye (λex=494 nm, λem=516 nm). Preloaded with Fuo-4 AM, a very bright fluorescence image is observed. In a parallel experiment with fluo-3 AM-loaded cells, the resulting fluorescence image, although clearly discernable in this case, is less bright.Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Count the cells and take 106 cells from each sample (control and experiment/s). 2. Collect the cells (5 min, 3000×g, 4 °C) and wash once in PBS. 3. Resuspend the cells in 0.5-ml PBS and add 0.5 μl of Fluo-4-AM (1 mM stock) to a final concentration of μM. Incubate at 37 °C for 1 h. 4. Wash the cells three times (2 min, 3000×g) with PBS and finally resuspend in 1-ml PBS. Separate into 2 flow cytometry tubes—0.5 ml in each. 5. Evaluate the staining by flow cytometry and analyze the data by a software such as CellQuest software.
Products are for research use only. Not for human use.
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