Description
DAPI dihydrochloride (4',6-diamidino-2-phenylindole) is a fluorescent stain by binding in the minor grove of A-T rich sequences of DNA.
Product information
CAS Number: 28718-90-3
Molecular Weight: 350.25
Formula: C16H17Cl2N5
Chemical Name: 2-(4-carbamimidoylphenyl)-1H-indole-6-carboximidamide dihydrochloride
Smiles: Cl.Cl.NC(=N)C1=CC2NC(=CC=2C=C1)C1C=CC(=CC=1)C(N)=N
InChiKey: FPNZBYLXNYPRLR-UHFFFAOYSA-N
InChi: InChI=1S/C16H15N5.2ClH/c17-15(18)10-3-1-9(2-4-10)13-7-11-5-6-12(16(19)20)8-14(11)21-13;;/h1-8,21H,(H3,17,18)(H3,19,20);2*1H
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 75 mg/mL (214.13 mM; Need ultrasonic). H2O : 8.33 mg/mL (23.78 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
DAPI (4',6-diamidino-2-phenylindole) is a DNA-specific probe which forms a fluorescent complex by attaching in the minor grove of A-T rich sequences of DNA. It also forms nonfluorescent intercalative complexes with double-stranded nucleic acids. DAPI can be used as a DNA-specific probe for flow cytometry, chromosome staining, DNA visualization and quantitation in histochemistry and biochemistry.
Products are for research use only. Not for human use.
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