Itaconate-alkyne


Catalog No. Size PriceQuantity
M25703-C Contact sales@xcessbio.com for quotation $100Unavailable

Description

Itaconate-alkyne (ITalk) is a specific bioorthogonal probe for quantitative and site-specific chemoproteomic profiling of Itaconation in living cells. Itaconate-alkyne, a functional analogue of Itaconate, exhibits comparable antiinflammatory effect with Itaconate and enables the labeling of bona fide targets of Itaconate.

Product information

CAS Number: 2454181-83-8

Molecular Weight: 238.28

Formula: C13H18O4

Chemical Name: 2-methylidene-4-(oct-7-yn-1-yloxy)-4-oxobutanoic acid

Smiles: C=C(CC(=O)OCCCCCCC#C)C(O)=O

InChiKey: NPWMZOZWVAFQMP-UHFFFAOYSA-N

InChi: InChI=1S/C13H18O4/c1-3-4-5-6-7-8-9-17-12(14)10-11(2)13(15)16/h1H,2,4-10H2,(H,15,16)

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : 250 mg/mL (1049.19 mM; Need ultrasonic).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Itaconate-alkyne (ITalk; 0.005, 0.01, 0.05, 0.1, 0.5, 1, 5, 10 mM; 0-4 hours) has the concentration- and time-dependent labeling in the lysates of Raw264.7 cells. Itaconate-alkyne (100 μM; for 4 hours) significantly increases the protein level of NRF-2 in LPS (100 ng/mL)-stimulated Raw264.7 cells and significantly inhibits lactate production in LPS-stimulated Raw264.7 cells. Itaconate-alkyne significantly inhibits the secretion of interlukin-1β (IL-1β) in LPS-timulated Raw264.7 cells. Itaconate-alkyne (100 μM; for 12 hours) does not affect the cell viability of Raw264.7 cells. Itaconate-alkyne can capture itaconate targets including DNA damage-binding protein 1 (DDB1), Gelsolin (GSN), ATP-citrate synthase (ACLY) and Adenylate kinase 2 (AK2) in inflammatory macrophages.

Products are for research use only. Not for human use.

Payment & Security

PayPal Venmo

Your payment information is processed securely. We do not store credit card details nor have access to your credit card information.

You may also like

Recently viewed