DAF-2DA


Catalog No. Size PriceQuantity
M25727-C Contact sales@xcessbio.com for quotation $100Unavailable

Description

DAF-2DA (5,6-Diaminofluorescein diacetat) is most widely probe for NO measurement.

Product information

CAS Number: 205391-02-2

Molecular Weight: 446.41

Formula: C24H18N2O7

Chemical Name: 3'-(acetyloxy)-5,6-diamino-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthen]-6'-yl acetate

Smiles: CC(=O)OC1C=C2OC3C=C(C=CC=3C3(OC(=O)C4=CC(N)=C(N)C=C34)C2=CC=1)OC(C)=O

InChiKey: PTSUYDXEEKDBQU-UHFFFAOYSA-N

InChi: InChI=1S/C24H18N2O7/c1-11(27)30-13-3-5-16-21(7-13)32-22-8-14(31-12(2)28)4-6-17(22)24(16)18-10-20(26)19(25)9-15(18)23(29)33-24/h3-10H,25-26H2,1-2H3

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Nitric oxide is a free radical signal molecule. Various methods are available for measurement of NO. Out of all methods, fluorescent probes to localize NO is very widely used method. Diaminofl uorescein in diacetate form (DAF-2DA) is most widely probe for NO measurement. This method is based on application of 4,5-diaminofl uorescein diacetate (DAF-2DA) which is actively diffused into cells, once taken up by cells cytoplasmic esterases cleave the acetate groups to generate 4,5-diaminofl uorescein; DAF-2. The generated DAF-2 can readily react with N2O3, which is an oxidation product of NO to generate the highly fluorescent DAF-2T (triazolofl uorescein).

Products are for research use only. Not for human use.

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