Description
L-Leucine-7-amido-4-methylcoumarin (Leu-AMC) hydrochloride is a bright blue fluorogenic peptidyl substrate for LAP3 (leucine aminopeptidase). L-Leucine-7-amido-4-methylcoumarin hydrochloride can be used for leucine aminopeptidase inhibition assays in vitro.
Product information
CAS Number: 62480-44-8
Molecular Weight: 324.80
Formula: C16H21ClN2O3
Chemical Name: (2S)-2-amino-4-methyl-N-(4-methyl-2-oxo-2H-chromen-7-yl)pentanamide hydrochloride
Smiles: Cl.CC1=CC(=O)OC2=CC(=CC=C12)NC(=O)[C@@H](N)CC(C)C
InChiKey: VCRXITKKWBOQRZ-ZOWNYOTGSA-N
InChi: InChI=1S/C16H20N2O3.ClH/c1-9(2)6-13(17)16(20)18-11-4-5-12-10(3)7-15(19)21-14(12)8-11;/h4-5,7-9,13H,6,17H2,1-3H3,(H,18,20);1H/t13-;/m0./s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
In leucine aminopeptidases inhibition assays in KB 3.1 cells. Compounds 3 and 5 are observed to weakly inhibit the biofilm formation by S. aureus and constrain inhibitory activity of ʟ-Leu-AMC hydrolysis in KB 3.1 cells. The amount of hydrolyzed 7-amino-4-methylcoumarin (AMC) is determined in a fluorescence spectrophotometer (excitation/emission: 365/440 nm).
Products are for research use only. Not for human use.
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