Description
RT-AM is a pro-drug real thiol. Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.
Product information
CAS Number: 2280796-94-1
Molecular Weight: 555.49
Formula: C26H25N3O11
Chemical Name: (acetyloxy)methyl 2-[(2E)-N-{2-[(acetyloxy)methoxy]-2-oxoethyl}-3-[7-(azetidin-1-yl)-2-oxo-2H-chromen-3-yl]-2-cyanoprop-2-enamido]acetate
Smiles: CC(=O)OCOC(=O)CN(CC(=O)OCOC(C)=O)C(=O)/C(=C/C1=CC2=CC=C(C=C2OC1=O)N1CCC1)/C#N
InChiKey: CLCCMBNDDZVBCA-AWQFTUOYSA-N
InChi: InChI=1S/C26H25N3O11/c1-16(30)36-14-38-23(32)12-29(13-24(33)39-15-37-17(2)31)25(34)20(11-27)9-19-8-18-4-5-21(28-6-3-7-28)10-22(18)40-26(19)35/h4-5,8-10H,3,6-7,12-15H2,1-2H3/b20-9+
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
To enhance the cell permeability of Real Thiol, the carboxylic acid groups is converted to acetoxymethyl (AM) esters, which are readily hydrolysed by esterases to regenerate Real Thiol inside cells. For cells treated with the RT-AM, real-time ratiometric images of the rapid changes of intracellular GSH concentrations in single cells can be generated by dividing the fluorescence intensity values for the 405 nm channel by the 488 nm channel at each corresponding pixel.
Products are for research use only. Not for human use.
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