Description
HADA hydrochloride (HCC-Amino-D-alanine hydrochloride) is a blue (λem~450 nm) fluorescent D-amino acid (FDAA). FDAAs are efficiently incorporated into the peptidoglycans (PGs) of diverse bacterial species at the sites of PG biosynthesis, allowing specific and covalent probing of bacterial growth with minimal perturbation.
Product information
CAS Number: 2253733-10-5
Molecular Weight: 328.71
Formula: C13H13ClN2O6
Chemical Name: (2R)-2-amino-3-[(7-hydroxy-2-oxo-2H-chromen-3-yl)formamido]propanoic acid hydrochloride
Smiles: Cl.N[C@H](CNC(=O)C1=CC2=CC=C(O)C=C2OC1=O)C(O)=O
InChiKey: PLVOEKQNBIXHDI-SBSPUUFOSA-N
InChi: InChI=1S/C13H12N2O6.ClH/c14-9(12(18)19)5-15-11(17)8-3-6-1-2-7(16)4-10(6)21-13(8)20;/h1-4,9,16H,5,14H2,(H,15,17)(H,18,19);1H/t9-;/m1./s1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Solubility: DMSO : 125 mg/mL (380.27 mM; Need ultrasonic).
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
FDAA labeling can take as little as 30s for a rapidly growing species such as Escherichia coli. Although HADA hydrochloride is dimmer and less photostable than FDL or TDL, it most reproducibly and robustly labels the PG of most bacterial species, typically without the need for extensive optimization.
Products are for research use only. Not for human use.
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