Real Thiol

Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells.

Product information

CAS Number: 2280796-90-7

Molecular Weight: 411.36

Formula: C20H17N3O7

Chemical Name: 2-[(2E)-3-[7-(azetidin-1-yl)-2-oxo-2H-chromen-3-yl]-N-(carboxymethyl)-2-cyanoprop-2-enamido]acetic acid

Smiles: N#C/C(=C\C1=CC2=CC=C(C=C2OC1=O)N1CCC1)/C(=O)N(CC(O)=O)CC(O)=O

InChiKey: VNKDTNMOOIIYSL-VGOFMYFVSA-N

InChi: InChI=1S/C20H17N3O7/c21-9-14(19(28)23(10-17(24)25)11-18(26)27)7-13-6-12-2-3-15(22-4-1-5-22)8-16(12)30-20(13)29/h2-3,6-8H,1,4-5,10-11H2,(H,24,25)(H,26,27)/b14-7+

Technical Data

Appearance: Solid Power

Purity: ≥98% (or refer to the Certificate of Analysis)

Solubility: DMSO : ≥ 101 mg/mL (245.53 mM).

Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis

Storage Condition: Dry, dark and -20 ^oC for 1 year or refer to the Certificate of Analysis.

Shelf Life: ≥12 months if stored properly.

Stock Solution Storage: 0 - 4 ^oC for 1 month or refer to the Certificate of Analysis.

Drug Formulation: To be determined

HS Tariff Code: 382200

How to use

In Vitro:

Real Thiol (RT) shows ratiometric fluorescence responses with a wide dynamic range when reacting with Glutathione (GSH). Real Thiol and its GSH adduct (RT-GSH) shows fluorescence maxima at 487 and 562 nm with excitation wavelengths at 405 and 488 nm, respectively. The Kd for the reaction between Real Thiol and GSH is 3.7 mM. Real Thiol has much improved quantum yields and photostability. The wide dynamic range of Real Thiol allows for monitoring of the GSH level changes within 1 to 10 mM in both directions in living cells. Gel permeation chromatography (GPC) analysis showes that 10% of Real Thiol reacts with thiolated proteins while 90% of Real Thiol reacts with GSH. Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs). 1. Real Thiol (RT) is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use. 2. Cells are cultured in 10% FBS DMEM containing different concentrations of Buthionine sulfoximine (0.9, 1.9, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 and 500 μM) for 72 h and harvested for analysis. 3. Cells are then suspended in fresh medium containing 1 μM of Real Thiol probe as single-cell suspensions, and keep the staining time between 9 and 10 min for each sample. 4. Analyze sample on a flow cytometer. PE and pacific-blue channels were selected to measure the fluorescence intensities of unreacted and reacted Real Thiol, respectively.

Products are for research use only. Not for human use.