Description
TMRM is a cell-permeant cationic lipophilic red fluorescent dye (λex=530 nm, λem=592 nm).
Product information
CAS Number: 115532-49-5
Molecular Weight: 401.48
Formula: C25H25N2O3
Chemical Name: 6-(dimethylamino)-9-[2-(methoxycarbonyl)phenyl]-N,N-dimethyl-3H-xanthen-3-iminium
Smiles: COC(=O)C1=CC=CC=C1C1C2=CC=C(C=C2OC2=CC(C=CC2=1)=[N+](C)C)N(C)C
InChiKey: WAWRKBQQBUDAMY-UHFFFAOYSA-N
InChi: InChI=1S/C25H25N2O3/c1-26(2)16-10-12-20-22(14-16)30-23-15-17(27(3)4)11-13-21(23)24(20)18-8-6-7-9-19(18)25(28)29-5/h6-15H,1-5H3/q+1
Technical Data
Appearance: Solid Power
Purity: ≥98% (or refer to the Certificate of Analysis)
Shipping Condition: Shipped under ambient temperature as non-hazardous chemical or refer to Certificate of Analysis
Storage Condition: Dry, dark and -20 oC for 1 year or refer to the Certificate of Analysis.
Shelf Life: ≥12 months if stored properly.
Stock Solution Storage: 0 - 4 oC for 1 month or refer to the Certificate of Analysis.
Drug Formulation: To be determined
HS Tariff Code: 382200
How to use
In Vitro:
TMRM is a fluorescent probe (excitation, 530±21 nm; emission, 592±22 nm). The fluorescence signal in the presence of safranin or TMRM shows a slight decrease after the addition of glutamate, indicative of increased polarization of the mitochondrial inner membrane. In the presence of TMRM (2 μM) the coupled respiration with Complex I substrates or upon the addition of Complex II substrate is decreased by 27%. Exposure of hippocampal cultures to low concentrations of TMRM (50 to 500 nM) for 1 to 3 hours results in selective staining of mitochondria in both neurons and the underlying glial cells. Exposure of hippocampal cultures to high concentrations of TMRM (1 to 25 µM) stains mitochondria selectively and quickly, reaching a plateau after 5 to 10 min. Low concentrations of TMRM (50 to 200 nM) do not induce apoptosis, whereas higher concentrations (0.5 and 2.5 µM) enhance apoptosis (KD = 500 nM).
Products are for research use only. Not for human use.
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